A Photo-Crosslinkable Kidney ECM-Derived Bioink Accelerates Renal Tissue Formation
A Photo-Crosslinkable Kidney ECM-Derived Bioink Accelerates Renal Tissue Formation
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DOI：
10.1002/adhm.201800992
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发表时间：
2019-04-11
影响因子：
10
通讯作者：
Lee, Sang Jin
中科院分区：
文献类型：
--
作者：
Ali, Mohamed;Kumar, Anil P. R.;Lee, Sang Jin
组织工程中的3D生物打印技术旨在制造临床上适用的组织结构，以取代受损或患病的组织和器官。3D生物打印的主要先决条件之一是找到合适的生物墨水，为细胞的生长和成熟提供组织特有的微环境。在这方面，脱细胞细胞外基质(DECM)衍生的水凝胶由于能够继承天然ECM的内在线索而被认为是基于细胞的生物打印的生物墨水。在此，开发了一种可光交联的肾脏ECM衍生生物墨水(KdECMMA)，它可以为肾组织生物打印提供肾脏特有的微环境。猪的整个肾脏通过灌注法脱细胞，在酸性溶液中溶解，然后通过甲基丙烯酸化进行化学修饰。研制了一种基于KdECMMA的生物油墨，并对其流变性和印刷适宜性进行了评价。结果表明，在KdECMMA生物墨水中生物打印的人肾细胞具有很高的活性，并随着时间的推移而成熟。此外，生物打印的肾脏结构显示出天然肾组织的结构和功能特征。组织特异性ECM衍生生物墨水的潜力被证明为基于细胞的生物打印，可以促进细胞成熟，最终组织形成。
3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue-specific microenvironment supporting the cellular growth and maturation. In this respect, decellularized extracellular matrix (dECM)-derived hydrogels have been considered as bioinks for the cell-based bioprinting due to their capability to inherit the intrinsic cues from native ECM. Herein, a photo-crosslinkable kidney ECM-derived bioink (KdECMMA) is developed that could provide a kidney-specific microenvironment for renal tissue bioprinting. Porcine whole kidneys are decellularized through a perfusion method, dissolved in an acid solution, and chemically modified by methacrylation. A KdECMMA-based bioink is formulated and evaluated for rheological properties and printability for the printing process. The results show that the bioprinted human kidney cells in the KdECMMA bioink are highly viable and mature with time. Moreover, the bioprinted renal constructs exhibit the structural and functional characteristics of the native renal tissue. The potential of the tissue-specific ECM-derived bioink is demonstrated for cell-based bioprinting that could enhance the cellular maturation and eventually tissue formation.