Tight junction-associated proteins (occludin, ZO-1, claudin-1, claudin-4) in squamous cell carcinoma and Bowen's disease
Tight junction-associated proteins (occludin, ZO-1, claudin-1, claudin-4) in squamous cell carcinoma and Bowen's disease
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DOI：
10.1111/j.1365-2133.2004.06029.x
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发表时间：
2004-08-01
影响因子：
10.3
通讯作者：
Miyachi, Y
中科院分区：
文献类型：
--
作者：
Morita, K;Tsukita, S;Miyachi, Y
背景表皮是典型的复层上皮，与单层上皮一样，在颗粒层中具有紧密连接（TJ）。迄今为止，在单纯性上皮肿瘤中已有TJ异常和claudin-1参与的报道。 目的 检测TJ相关蛋白（occludin、ZO-1、claudin-1和claudin-4）在正常人表皮和恶性角化疾病中的表达。方法 正常人表皮、5例鳞状上皮肿瘤中蛋白的表达 对皮肤细胞癌(SCC)和5例鲍文病(BD)进行免疫荧光染色检查。结果在正常人表皮中，occludin、ZO-1和claudin-4在颗粒层的细胞-细胞边界特异性或显性表达，而claudin-1在整个上皮中表达。在SCC中，occludin、ZO-1、claudin-1和claudin-4在角化的肿瘤细胞（如癌珍珠）中强烈表达。 Claudin-1在未角化的肿瘤细胞中异质表达，而occludin、ZO-1和claudin-4的表达减少或缺失。在BD中，除了在正常表皮中观察到的表达模式外，在细胞-细胞边界还观察到occludin、ZO-1、claudin-1和claudin-4的异常表达。结论occludin、ZO-1和claudin-4的表达与SCC和BD中的角化相关。然而，claudin-1在SCC中的异质表达不仅仅由角化决定。
Background The epidermis, which is a typical stratified epithelium, has tight junctions (TJs) in the granular layer, as do simple epithelia. So far, abnormalities of TJs and involvement of claudin-1 have been reported in tumours of simple epithelia.Objectives To examine the expression of TJ-associated proteins (occludin, ZO-1, claudin-1 and claudin-4) in normal human epidermis and in malignant disorders of keratinization.Methods Expression of the proteins in normal human epidermis, five cases of squamous cell carcinoma (SCC) of the skin and five cases of Bowen's disease (BD) was examined by immunofluorescence staining.Results In normal human epidermis, occludin, ZO-1 and claudin-4 were expressed at the cell-cell borders in the granular layer specifically or dominantly, whereas claudin-1 was expressed in the whole epithelium. In SCC, occludin, ZO-1, claudin-1 and claudin-4 were strongly expressed in tumour cells with keratinization such as cancer pearls. Claudin-1 was heterogeneously expressed in unkeratinized tumour cells, whereas expression of occludin, ZO-1 and claudin-4 was decreased or absent. In BD, aberrant expression of occludin, ZO-1, claudin-1 and claudin-4 was observed at the cell-cell borders in addition to their expression patterns observed in normal epidermis.Conclusions Expression of occludin, ZO-1 and claudin-4 is associated with keratinization in SCC and BD. However, the heterogeneous expression of claudin-1 in SCC is not determined only by keratinization.