Cellulosome Complexes: Natural Biocatalysts as Arming Microcompartments of Enzymes
Cellulosome Complexes: Natural Biocatalysts as Arming Microcompartments of Enzymes
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DOI：
10.1159/000351358
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发表时间：
2013-01-01
影响因子：
1.2
通讯作者：
Ueda, Mitsuyoshi
中科院分区：
文献类型：
--
作者：
Bae, Jungu;Morisaka, Hironobu;Ueda, Mitsuyoshi
纤维素是木质纤维素生物质的主要组分，是自然界中最丰富的碳水化合物聚合物。已知只有有限数量的微生物降解纤维素，由于其晶体结构，纤维素是高度可降解的。厌氧细菌通过产生纤维素体有效地降解纤维素，纤维素体是纤维素酶与支架蛋白结合的复合物。负责纤维素体的组装和效率的潜在机制尚未完全理解。已经广泛研究了粘着蛋白-锚定蛋白特异性以理解多纤维素体组装。此外，蛋白质组学的最新进展，使纤维素体系统的生长底物依赖性的变化的整体分析。此外，使用设计的微纤维素体研究了纤维素体协同作用的邻近效应和靶向效应。本文综述了纤维素酶体组装、优化纤维素酶体生产的策略以及纤维素酶体作为微生物细胞表面微区室的有益特征。版权所有（C）2013 S. Karger AG，巴塞尔
Cellulose, a primary component of lignocellulosic biomass, is the most abundant carbohydrate polymer in nature. Only a limited number of microorganisms are known to degrade cellulose, which is highly recalcitrant due to its crystal structure. Anaerobic bacteria efficiently degrade cellulose by producing cellulosomes, which are complexes of cellulases bound to scaffoldins. The underlying mechanisms that are responsible for the assembly and efficiency of cellulosomes are not yet fully understood. The cohesin-dockerin specificity has been extensively studied to understand cellulosome assembly. Moreover, the recent progress in proteomics has enabled integral analyses of the growth-substrate-dependent variations in cellulosomal systems. Furthermore, the proximity and targeting effects of cellulosomal synergistic actions have been investigated using designed minicellulosomes. The recent findings about cellulosome assembly, strategies for optimal cellulosome production, and beneficial features of cellulosomes as an arming microcompartment on the microbial cell surface are summarized here. Copyright (C) 2013 S. Karger AG, Basel