Sample Preparation: A Crucial Factor for the Analytical Performance of Rationally Designed MALDI Matrices
Sample Preparation: A Crucial Factor for the Analytical Performance of Rationally Designed MALDI Matrices
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DOI：
10.1021/ac504412p
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发表时间：
2015-02-03
影响因子：
7.4
通讯作者：
Cramer, Rainer
中科院分区：
文献类型：
--
作者：
Wiangnon, Kanjana;Cramer, Rainer
有证据表明，合理设计的MALDI基质4-氯-a-氰肉桂酸（ClCCA）与其完善的前代α -氰基-4-羟基肉桂酸（CHCA）相比，其性能显著依赖于样品制备，如靶板的选择。在这种情况下，很明显，MALDI基质的任何合理设计及其成功应用都必须考虑更多的物理化学参数，除了基本（溶液和/或气相）化学参数外，还包括样品结晶和形态/拓扑。
Evidence is presented that the performance of the rationally designed MALDI matrix 4-chloro-a-cyanocinnamic acid (ClCCA) in comparison to its well-established predecessor alpha-cyano-4-hydroxycinnamic acid (CHCA) is significantly dependent on the sample preparation, such as the choice of the target plate. In this context, it becomes clear that any rational designs of MALDI matrices and their successful employment have to consider a larger set of physicochemical parameters, including sample crystallization and morphology/topology, in addition to parameters of basic (solution and/or gas-phase) chemistry.