TbRGG1, an essential protein involved in kinetoplastid RNA metabolism that is associated with a novel multiprotein complex
TbRGG1, an essential protein involved in kinetoplastid RNA metabolism that is associated with a novel multiprotein complex
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DOI：
10.1261/rna.888808
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发表时间：
2008-05-01
期刊：
影响因子：
4.5
通讯作者：
Lukes, Julius
中科院分区：
文献类型：
--
作者：
Hashimi, Hassan;Zikova, Alena;Lukes, Julius
动质体线粒体转录物的尿苷插入/缺失RNA编辑通过涉及许多蛋白质和多个蛋白质复合物的复杂机制进行。在这里，我们描述了沉默TbRGG 1基因的RNA干扰对RNA编辑的影响，在布氏锥虫的前循环阶段。TbRGG 1是细胞生长的必需蛋白，其缺乏导致编辑的mRNA的总体下降，而从未编辑的RNA的水平保持不变。TbRGG 1表达的抑制对20 S编辑体和MRP 1/2复合体没有影响。TbRGG 1的TAP标签纯化共分离出一种新的多蛋白复合物，其协会进一步验证了TAP标签分析的其他两个组成部分的复杂。TbRGG 1与该复合物的相互作用似乎是由RNA介导的。我们的研究结果表明，TbRGG 1蛋白在稳定编辑的RNA或编辑效率的功能，相关的新复合物可能在线粒体RNA代谢中发挥作用。我们暂时将其命名为线粒体RNA结合复合物1（put-MRB复合物1）。
The uridine insertion/deletion RNA editing of kinetoplastid mitochondrial transcripts is performed by complex machinery involving a number of proteins and multiple protein complexes. Here we describe the effect of silencing of TbRGG1 gene by RNA interference on RNA editing in procyclic stage of Trypanosoma brucei. TbRGG1 is an essential protein for cell growth, the absence of which results in an overall decline of edited mRNAs, while the levels of never-edited RNAs remain unaltered. Repression of TbRGG1 expression has no effect on the 20S editosome and MRP1/2 complex. TAP-tag purification of TbRGG1 coisolated a novel multiprotein complex, and its association was further verified by TAP-tag analyses of two other components of the complex. TbRGG1 interaction with this complex appears to be mediated by RNA. Our results suggest that the TbRGG1 protein functions in stabilizing edited RNAs or editing efficiency and that the associated novel complex may have a role in mitochondrial RNA metabolism. We provisionally name it putative mitochondrial RNA-binding complex 1 (put-MRB complex 1).