The LZTFL1 gene is a part of a transcriptional map covering 250 kb within the common eliminated region 1 (C3CER1) in 3p21.3
The LZTFL1 gene is a part of a transcriptional map covering 250 kb within the common eliminated region 1 (C3CER1) in 3p21.3
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DOI：
10.1006/geno.2000.6498
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发表时间：
2001-04-01
期刊：
影响因子：
4.4
通讯作者：
Dumanski, JP
中科院分区：
文献类型：
--
作者：
Kiss, H;Kedra, D;Dumanski, JP
3p上的缺失已经在大量的人类肿瘤中被描述，这表明存在一个肿瘤抑制基因(S)，我们先前利用消除试验，从人类3p21.3(C3CER1)中定义了一个1-Mb片段，基因组测序使我们能够构建一个包含5个基因的250kb的转录图谱。我们鉴定了人类亮氨酸拉链基因亮氨酸拉链转录因子样1(LZTFL1)及其小鼠同源基因(Lztfl1)，LZTPL1基因有两种转录物异构体，显示交替的多聚腺苷化，我们定位了酵母SAC2(肌动蛋白抑制因子)基因的人类同源基因，并对小鼠SAD基因进行了鉴定和定位。此外，还鉴定了XT3基因，该基因编码Na+/Cl-神经递质超家族成员。研究表明，XT3基因有一个选择性剪接的脑特异性异构体，预计将移除12个假定的跨膜结构域中的1个。该转录图谱还包括CC趋化因子受体9基因(CCR9)和含有LIM结构域的基因1(LIMD1)。这项工作部分定义了C3CER1的基因内容，这是描述其在肿瘤发生中的作用的先决条件。(C)2001年学术出版社。
Deletions on 3p have been described in a large number of human tumors, suggesting the presence of a tumor suppressor gene(s), Using the elimination test, we previously defined a 1-Mb segment from human 3p21.3 (C3CER1), Genomic sequencing allowed us to construct a transcription map covering 250 kb containing five genes. We have characterized a human leucine zipper containing gene, leucine zipper transcription factor-like 1 (LZTFL1), and its mouse orthologue (Lztfl1), which was also mapped to mouse chromosome 9F, The LZTPL1 gene has two transcript isoforms displaying alternative polyadenylation, We have localized the human orthologue of the yeast SAC2 (suppressor of actin) gene as well as characterized and mapped the mouse Sad gene. Furthermore, the XT3 gene was characterized, encoding a member of the Na+/Cl- neurotransmitter superfamily. It has been shown that the XT3 gene had an alternatively spliced brain-specific isoform, predicted to remove 1 of 12 putative transmembrane domains. The transcription map also includes the CC chemokine receptor 9 gene (CCR9) and the LIM domain containing gene 1 (LIMD1). This work partially defines the gene content of C3CER1 that is a prerequisite for delineation of its role in tumorigenesis. (C) 2001 Academic Press.