Allelotype analysis of adenocarcinoma of the gastric cardia.
Allelotype analysis of adenocarcinoma of the gastric cardia.
复制标题
胃CCADIA腺癌的化学型分析。
DOI：
10.1038/bjc.1997.578
复制
发表时间：
1997
影响因子：
8.8
通讯作者：
Russell, S E
中科院分区：
文献类型：
--
作者：
Gleeson, C M;Sloan, J M;McGuigan, J A;Ritchie, A J;Weber, J L;Russell, S E
为了确定参与近端胃腺癌发展的染色体位点，本研究描绘了一系列 38 例胃贲门腺癌中的等位基因失衡模式。总共分析了 137 个微卫星标记，涵盖所有常染色体臂（不包括近端着丝臂）。在所研究的总共 39 个染色体臂中，平均有 35 个对每位患者提供了信息。肿瘤组表现出高度的等位基因失衡，观察到的 29 种肠型腺癌的等位基因失衡中位数为 0.47，9 种弥漫型腺癌的中位等位基因失衡分数为 0.54。在许多染色体臂上的两种组织学亚型中，>50% 的信息病例中检测到等位基因不平衡。在肠道亚型中，包括 3p (61%)、4q (71%)、5q (59%)、8p (60%)、9p (65%)、9q (83%)、12q (52%)、13q (52%)、17p (78%) 和 18q (70%)。与肠型肿瘤相比，弥漫型肿瘤在 16q 染色体上检测到等位基因不平衡的发生率更高。对 4q 和 6q 染色体进行更详细的定位发现了许多具有亚染色体断点的病例。总之，该分析表明基因组区域可能参与近端胃癌的发展。
To identify chromosomal loci involved in the development of proximal gastric adenocarcinoma, this study delineated the pattern of allelic imbalance in a series of 38 adenocarcinomas arising in the gastric cardia. A total of 137 microsatellite markers covering all autosomal arms, excluding acrocentric arms, were analysed. A mean of 35 out of a total of 39 chromosomal arms studied were informative for each patient. The tumour group demonstrated a high level of allelic imbalance, with an observed median fractional allelic imbalance of 0.47 for the 29 intestinal-type adenocarcinomas and 0.54 for the nine diffuse-type adenocarcinomas. Allelic imbalance was detected in >50% of informative cases in both histological subtypes on a number of chromosomal arms. In the intestinal subtype, these included, 3p (61%), 4q (71%), 5q (59%), 8p (60%), 9p (65%), 9q (83%), 12q (52%), 13q (52%), 17p (78%) and 18q (70%). A higher incidence of allelic imbalance was detected on chromosome 16q in tumours of the diffuse type relative to those of the intestinal type. A more detailed mapping on chromosomes 4q and 6q identified a number of cases with subchromosomal breakpoints. In conclusion, this analysis has indicated regions of the genome potentially involved in the development of proximal gastric carcinomas.