Active microrheology and simultaneous visualization of sheared phospholipid monolayers.
Active microrheology and simultaneous visualization of sheared phospholipid monolayers.
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DOI：
10.1038/ncomms1321
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发表时间：
2011
影响因子：
16.6
通讯作者：
Squires, T. M.
中科院分区：
文献类型：
--
作者：
Choi, S. Q.;Steltenkamp, S.;Zasadzinski, J. A.;Squires, T. M.
表面活性物质的二维薄膜--从磷脂和蛋白质到纳米颗粒和胶体--稳定了流体界面，这对日常生活的科学、技术和工程至关重要。界面的2D特性带来了独特的挑战和机遇：2D薄膜和块体流体之间的耦合使表面动态特性的测量变得复杂，但允许在变形过程中直接显示界面微结构。在这里，我们提出了一种新的技术，它结合了主动微观流变学和荧光显微镜来可视化流体界面，当它们在外加应力下变形时，允许结构和流变学在微米尺度上在单层膜中相关联。我们发现，即使是简单的、单组分的脂单分子膜也可以表现出粘弹性、历史依赖性、屈服应力和数小时的弹性回弹和老化时间尺度。应力作用下单分子膜的同时可视化显示，丰富的动力学响应来自于液晶区及其边界的协同动力学和形变。二维流体界面是普遍存在的，但由于薄膜与体相流体之间的耦合，研究其表面动力学性质是困难的。Choi等人。结合主动微观流变学和荧光显微镜对施加应力下的流体界面进行成像。
Two-dimensional films of surface-active agents—from phospholipids and proteins to nanoparticles and colloids—stabilize fluid interfaces, which are essential to the science, technology and engineering of everyday life. The 2D nature of interfaces present unique challenges and opportunities: coupling between the 2D films and the bulk fluids complicates the measurement of surface dynamic properties, but allows the interfacial microstructure to be directly visualized during deformation. Here we present a novel technique that combines active microrheology with fluorescence microscopy to visualize fluid interfaces as they deform under applied stress, allowing structure and rheology to be correlated on the micron-scale in monolayer films. We show that even simple, single-component lipid monolayers can exhibit viscoelasticity, history dependence, a yield stress and hours-long time scales for elastic recoil and aging. Simultaneous visualization of the monolayer under stress shows that the rich dynamical response results from the cooperative dynamics and deformation of liquid-crystalline domains and their boundaries. Two-dimensional fluid interfaces are ubiquitous, but studying their surface dynamic properties is difficult because of coupling between the film and bulk fluid. Choi et al. combine active microrheology with fluorescence microscopy to image fluid interfaces under applied stress.
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影响因子：
8.6
作者：
Ding, JQ;Warriner, HE;Zasadzinski, JA
通讯作者：
Zasadzinski, JA
影响因子：
8.6
作者：
Britton, MM;Callaghan, PT
通讯作者：
Callaghan, PT
影响因子：
3.9
作者：
Lauger, J;Robertson, CR;Fuller, GG
通讯作者：
Fuller, GG
影响因子：
3.9
作者：
Lee, Myung Han;Lapointe, Clayton P.;Leheny, Robert L.
通讯作者：
Leheny, Robert L.
影响因子：
3.7
作者：
HUGHES, BD;PAILTHORPE, BA;WHITE, LR
通讯作者：
WHITE, LR