Radial shock waves effectively introduced NF‐kappa B decoy into rat achilles tendon cells in vitro
Radial shock waves effectively introduced NF‐kappa B decoy into rat achilles tendon cells in vitro
复制标题
DOI：
10.1002/jor.21081
复制
发表时间：
2010-08
影响因子：
2.8
通讯作者：
K. Sugioka;K. Nakagawa;Ryo Murata;N. Ochiai;T. Sasho;M. Arai;H. Tsuruoka;S. Ohtori;T. Saisu;T. Gemba;Kazuhisa Takahashi
中科院分区：
文献类型：
--
作者：
K. Sugioka;K. Nakagawa;Ryo Murata;N. Ochiai;T. Sasho;M. Arai;H. Tsuruoka;S. Ohtori;T. Saisu;T. Gemba;Kazuhisa Takahashi
本研究的目的是使用大鼠跟腱细胞测试径向冲击波是否可以增强核因子-κ B（NF-κB）诱饵寡脱氧核苷酸的引入，据报道，该寡脱氧核苷酸可显著抑制NF-κB活化并抑制促炎细胞因子基因表达。在培养基中存在NF-κB诱饵（用或不用异硫氰酸荧光素（FITC）标记）的情况下，在可变条件下对肌腱细胞施加径向冲击波并培养24 h。通过计数FITC阳性细胞评估转染率，并评估细胞中IL-1诱导的NF-κB活化。径向冲击波显著增强了NF-κB诱饵-FITC向肌腱细胞的引入。NF-κB诱骗物预处理结合放射状冲击波暴露可显著抑制IL-1诱导的NF-κB活化。本研究证明了径向冲击波对将NF-κB诱饵引入肌腱细胞的有效性。放射状冲击波治疗联合局部NF-κB诱饵给药可能是慢性肌腱病的一种新的治疗策略。© 2010骨科研究学会。由威利期刊公司出版J Orthop Res 28：1078-1083，2010
The purpose of this study was to test if radial shock waves could enhance the introduction of nuclear factor‐kappa B (NF‐κB) decoy oligodeoxynucleotides, which is reported to markedly inhibit NF‐κB activation and suppress pro‐inflammatory cytokine gene expression, using rat Achilles tendon cells. In the presence of NF‐κB decoy labeled with or without fluorescein isothiocyanate (FITC) in culture media, radial shock waves were applied to the tendon cells in variable conditions and cultivated for 24 h. The transfection rate was assessed by counting FITC‐positive cells, and IL‐1–induced NF‐κB activation in the cells was assessed. Radial shock waves significantly enhanced introduction of NF‐κB decoy‐FITC into the tendon cells. IL‐1–induced NF‐κB activation was significantly inhibited by pretreatment of the cells with NF‐κB decoy combined with radial shock wave exposure. The present study demonstrated the effectiveness of radial shock waves on introduction of NF‐κB decoy into tendon cells. Radial shock wave treatment combined with local NF‐κB decoy administration could be a novel therapeutic strategy for chronic tendinopathy. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1078–1083, 2010