Cell cycle arrest induced by inhibitors of epigenetic modifications in maize (Zea mays) seedling leaves: characterization of the process and possible mechanisms involved
Cell cycle arrest induced by inhibitors of epigenetic modifications in maize (Zea mays) seedling leaves: characterization of the process and possible mechanisms involved
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玉米幼苗叶片表观遗传修饰抑制剂诱导的细胞周期停滞：过程特征和可能的机制
DOI：
10.1111/nph.13942
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发表时间：
2016
期刊：
影响因子：
9.4
通讯作者：
Lijia Li
中科院分区：
文献类型：
--
作者：
Pu Wang;Hao Zhang;Haoli Hou;Qing Wang;Yingnan Li;Yingnan Li;Liangfu Xie;Fei Gao;Shibin He;Lijia Li
表观遗传修饰在染色质结构的调控中起重要作用，并参与细胞周期进程，包括有丝分裂和减数分裂。为了探索表观遗传修饰与细胞周期的关系，我们用6种不同的表观遗传修饰相关抑制剂处理玉米(Zea Mays)幼苗，通过流式细胞仪分析确定了合成后阶段(G2)的停滞。在不同处理下，总H4K5ac水平显著增加，H3S10ph信号的分布明显改变。对有丝分裂不同时期细胞的进一步统计证实，细胞周期停滞在前期。处理植株中过氧化氢的浓度相对较高，抗氧化剂硫脲可以抵消抑制剂的影响。此外，暴露3d后，所有处理植株的末端脱氧核苷酸转移酶介导的脱氧尿苷缺口末端标记(TUNEL)和γ-H2AX免疫染色结果均为阴性，此外，处理植株的拓扑异构酶基因的表达水平相对低于未处理植株.这些结果表明，这些表观遗传修饰的抑制剂可能通过抑制DNADNA拓扑异构酶基因的表达而导致前期停滞，并伴随着H4K5ac和H3S10ph组蛋白修饰的变化.
Epigenetic modifications play crucial roles in the regulation of chromatin architecture and are involved in cell cycle progression, including mitosis and meiosis..To explore the relationship between epigenetic modifications and the cell cycle, we treated maize (Zea mays) seedlings with six different epigenetic modification-related inhibitors and identified the postsynthetic phase (G2) arrest via flow cytometry analysis..Total H4K5ac levels were significantly increased and the distribution of H3S10ph signalling was obviously changed in mitosis under various treatments. Further statistics of the cells in different periods of mitosis confirmed that the cell cycle was arrested at preprophase. Concentrations of hydrogen peroxide were relatively higher in the treated plants and the antioxidant thiourea could negate the influence of the inhibitors. Moreover, all of the treated plants displayed negative results in the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling (TUNEL) and γ-H2AX immunostaining assays after exposure for 3 d. Additionally, the expression level of topoisomerase genes in the treated plants was relatively lower than that in the untreated plants..These results suggest that these inhibitors of epigenetic modifications could cause preprophase arrest via reactive oxygen species formation inhibiting the expression of DNA topoisomerase genes, accompanied by changes in the H4K5ac and H3S10ph histone modifications.