CDNAS ENCODING THE LARGE SUBUNIT OF HUMAN REPLICATION FACTOR-C
CDNAS ENCODING THE LARGE SUBUNIT OF HUMAN REPLICATION FACTOR-C
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DOI：
10.1073/pnas.90.23.11014
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发表时间：
1993-12-01
影响因子：
11.1
通讯作者：
STILLMAN, B
中科院分区：
文献类型：
--
作者：
BUNZ, F;KOBAYASHI, R;STILLMAN, B
复制因子 C (RFC) 是一种多亚基 DNA 聚合酶辅助蛋白，在猿猴病毒 40 DNA 体外复制过程中协调合成两条 DNA 链所需。先前的研究表明，RFC 是一种 DNA 依赖性 ATP 酶，以结构特异性方式结合到与模板 DNA 杂交的引物的 3' 末端，这种活性被认为是该多亚基复合物的 140 kDa 成分所固有的。在此，描述了编码该亚基的 cDNA 的分离和分析。对全长编码序列的分析揭示了一个 3.4 kb 的开放阅读框，编码 1148 个氨基酸的蛋白质，预测分子量为 130 kDa。观察到一个假定的 ATP 结合基序，该基序与 RFC 的几个较小亚基以及细菌和病毒来源的功能同源复制因子中的基序相似。这些蛋白质中还保留着“死亡”盒。预测的蛋白质与小鼠来源的 DNA 结合蛋白显示出显着的同一性（B. Luckow、P. Lichter 和 G. Schutz，个人通讯）。在 RFC、聚（ADP-核糖）聚合酶和细菌 DNA 连接酶的 140 kDa 亚基的氨基酸序列之间也发现了相似区域，这可能代表了这些结合相似 DNA 底物的蛋白质的保守结构特征。
Replication factor C (RFC) is a multisubunit, DNA polymerase accessory protein required for the coordinated synthesis of both DNA strands during simian virus 40 DNA replication in vitro. Previous studies have shown that RFC is a DNA-dependent ATPase that binds in a structure-specific manner to the 3' end of a primer hybridized to a template DNA, an activity thought intrinsic to the 140-kDa component of this multisubunit complex. Here, the isolation and analysis of cDNAs encoding this subunit is described. Analysis of the full-length coding sequence revealed an open reading frame of 3.4 kb, encoding an 1148-amino acid protein with a predicted molecular mass of 130 kDa. A putative ATP-binding motif was observed that is similar to a motif in several of the smaller subunits of RFC and in functionally homologous replication factors of bacterial and viral origin. A ''DEAD'' box is also conserved among these proteins. The predicted protein shows significant identity with a DNA-binding protein of murine origin (B. Luckow, P. Lichter, and G. Schutz, personal communication). Regions of similarity were also seen between the amino acid sequences of the 140-kDa subunit of RFC, poly(ADP-ribose) polymerase, and bacterial DNA ligases-possibly representing a conserved structural feature of these proteins that bind similar DNA substrates.