A hypothesis for DNA viruses as the origin of eukaryotic replication proteins
A hypothesis for DNA viruses as the origin of eukaryotic replication proteins
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DOI：
10.1128/jvi.74.15.7079-7084.2000
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发表时间：
2000-08-01
影响因子：
5.4
通讯作者：
DeFilippis, VR
中科院分区：
文献类型：
--
作者：
Villarreal, LP;DeFilippis, VR
真核复制DNA聚合酶与真核和细菌T4噬菌体的大型DNA病毒相似，但与真细菌不同。我们提出并检验了这样的假设：DNA 病毒复制蛋白在进化过程中产生了真核生物的复制蛋白。我们选择藻DNA病毒（感染微藻）的DNA聚合酶作为分析的基础，因为它代表原始真核生物的病毒。我们证明它与真核生物及其某些大型 DNA 病毒的复制 DNA 聚合酶具有显着的相似性。序列比对证实了这种相似性，并确定了聚合酶氨基末端存在高度保守的结构域。随后的系统发育树重建表明，这些藻类病毒 DNA 聚合酶位于包含所有真核 DNA 聚合酶 δ 成员的进化枝的根部附近，但该进化枝不包含其他 DNA 病毒的聚合酶。我们考虑了关于这种关系极性的争论，并提出了这样的假设：DNA 病毒的复制基因产生了真核生物的复制基因，而不是相反的方向。
The eukaryotic replicative DNA polymerases are similar to those of large DNA viruses of eukaryotic and bacterial T4 phages but not to those of eubacteria. We develop and examine the hypothesis that DNA virus replication proteins gave rise to those of eukaryotes during evolution. We chose the DNA polymerase from phycodnavirus (which infects microalgae) as the basis of this analysis, as it represents a virus of a primitive eukaryote. We show that it has significant similarity with replicative DNA polymerases of eukaryotes and certain of their large DNA viruses. Sequence alignment confirms this similarity and establishes the presence of highly conserved domains in the polymerase amino terminus. Subsequent reconstruction of a phylogenetic tree indicates that these algal viral DNA polymerases are near the root of the clade containing all eukaryotic DNA polymerase delta members but that this clade does not contain the polymerases of other DNA viruses. We consider arguments for the polarity of this relationship and present the hypothesis that the replication genes of DNA viruses gave rise to those of eukaryotes and not the reverse direction.