cfNOMe- A single assay for comprehensive epigenetic analyses of cell-free DNA
cfNOMe- A single assay for comprehensive epigenetic analyses of cell-free DNA
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DOI：
10.1186/s13073-020-00750-5
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发表时间：
2020-06-24
期刊：
影响因子：
12.3
通讯作者：
Altmueller, Janine
中科院分区：
文献类型：
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作者：
Erger, Florian;Noerling, Deborah;Altmueller, Janine
无细胞DNA（cfDNA）分析在癌症诊断和产前检测中变得至关重要。我们提出了cfNOMe，这是一种在单一测定中使用非破坏性酶促胞嘧啶转化和定制生物信息学管道测量cfDNA胞嘧啶甲基化和核小体占用的二合一方法。我们表明，酶促胞嘧啶转化比亚硫酸氢盐转化更好地保留了cfDNA片段化信息。虽然以前需要单独的实验来研究表观遗传标记，但cfNOMe为两者提供了可靠的结果，使得能够进行更全面和更便宜的表观遗传cfDNA谱分析。cfNOMe具有在具有cfDNA组成的系统性扰动的疾病中推进生物标志物发现和诊断用途的潜力。
Cell-free DNA (cfDNA) analysis has become essential in cancer diagnostics and prenatal testing. We presentcfNOMe, a two-in-one method of measuring cfDNA cytosine methylation and nucleosome occupancy in a single assay using non-disruptive enzymatic cytosine conversion and a custom bioinformatic pipeline. We show that enzymatic cytosine conversion better preserves cfDNA fragmentation information than does bisulfite conversion. Whereas previously separate experiments were required to study either epigenetic marking,cfNOMedelivers reliable results for both, enabling more comprehensive and inexpensive epigenetic cfDNA profiling.cfNOMehas the potential to advance biomarker discovery and diagnostic usage in diseases with systemic perturbations of cfDNA composition.