CROSS-REACTIVITY IN SEROLOGICAL TESTS FOR LYME-DISEASE AND OTHER SPIROCHETAL INFECTIONS
CROSS-REACTIVITY IN SEROLOGICAL TESTS FOR LYME-DISEASE AND OTHER SPIROCHETAL INFECTIONS
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DOI：
10.1093/infdis/156.1.183
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发表时间：
1987-07-01
影响因子：
6.4
通讯作者：
JOHNSON, RC
中科院分区：
文献类型：
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作者：
MAGNARELLI, LA;ANDERSON, JF;JOHNSON, RC
为了评价间接荧光抗体(IFA)试验、酶联免疫吸附试验(EL ISA)和镜检凝集试验(MA)的特异性，对163例莱姆病、虱传或虱传复发热、雅司病、梅毒、钩端螺旋体病或落基山斑点热患者的血清标本进行了分析。当莱姆病、壁虱传播复发热和虱传复发热患者的血清与异种疏螺旋体抗原进行检测时，发生了强烈的交叉反应。梅毒免疫荧光试验中与梅毒螺旋体结合的伯氏疏螺旋体抗体。梅毒患者的血清在IFA试验和莱姆病的ELISA法中有交叉反应。然而，在MA或IFA试验中，针对疏螺旋体或螺旋体的免疫球蛋白抗体不与问号钩端螺旋体的血清型反应，在相互分析中假阳性的发生率可以忽略不计。
Serum specimens from 163 persons with Lyme disease, tick-borne or louse-borne relapsing fever, yaws, syphilis, leptospirosis, or Rocky Mountain spotted fever were analyzed to assess the specificity of indirect fluorescent antibody (IFA) tests, an enzyme-linked immunosorbent assay (ELISA), and microscopic agglutination (MA) procedures. Strong cross-reactivity occurred when sera from individuals with Lyme disease, tick-borne relapsing fever, and louse-borne relapsing fever were tested against heterologous Borrelia antigens. Antibodies to Borrelia burgdorferi bound to Treponema pallidum in immunofluorescence tests for syphilis. Sera from subjects with syphilis cross-reacted in IFA tests and the ELISA for Lyme disease. Immunoglobulin antibodies to Borrelia or Treponema spirochetes, however, did not react with serovars of Leptospira interrogans in MA or IFA tests, and the prevalence of false-positive in the reciprocal analyses was negligible.