Nanopore single-molecule detection of Bleomycin via dumbbell DNA scission
Nanopore single-molecule detection of Bleomycin via dumbbell DNA scission
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DOI：
10.1016/j.microc.2021.106738
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发表时间：
2021-08
影响因子：
4.8
通讯作者：
Tingying Li;Xinying Li;Xiao-xiao Li;Li Yang;Hailong Wang
中科院分区：
文献类型：
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作者：
Tingying Li;Xinying Li;Xiao-xiao Li;Li Yang;Hailong Wang
随着博莱霉素在临床上的广泛应用，建立简便、灵敏的博莱霉素定量检测方法具有重要意义。基于BLM对DNA的断裂作用，提出了一种在纳米孔传感器平台上以哑铃状DNA为探针检测BLM的新方法。选择具有TTTTT-五碱基环的哑铃形DNA，因为其在纳米孔中的停留时间更长且堵塞频率更高。随着BLM·Fe（II）的加入，哑铃型DNA的阻断事件逐渐转化为单链DNA（ssDNA）片段信号，并且在一定范围内，事件间隔（τon）与BLM浓度之间存在定量关系，具有高选择性和0.1 nM的超低检测限，是一种很有前途的定量检测BLM的方法。
It’s of great significance to develop convenient and sensitive quantitative detection assays for the bleomycins (BLMs) as the BLMs are widely used in clinical treatment. In the present work, the authors described a new method for BLM detection using dumbbell DNA as probe on the nanopore sensor platform, which is based on the scission function on DNA by BLM. A dumbbell DNA with TTTTT-five bases loop was chosen due to its longer dwell time and higher frequency of the blockages in nanopore. With addition of BLM·Fe(II), the blockage events of the dumbbell DNA gradually transfer to single strand DNA (ssDNA) fragment signals, and it turns out to be a quantitative relationship between event intervals (τon) and the concentration of BLM in a certain range, making it a promising method for quantitative detection of BLMs with high selectivity and ultra-low detection limit of 0.1 nM.