Time course of programmed cell death, which included autophagic features, in hybrid tobacco cells expressing hybrid lethality
Time course of programmed cell death, which included autophagic features, in hybrid tobacco cells expressing hybrid lethality
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表达混合致死性的杂交烟草细胞中程序性细胞死亡的时间过程，包括自噬特征
DOI：
10.1007/s00299-016-2048-1
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发表时间：
2016
影响因子：
6.2
通讯作者：
Tetsuya Yamada
中科院分区：
文献类型：
--
作者：
Naoya Ueno;Saori Nihei;Naoto Miyakawa;Tadashi Hirasawa;Motoki Kanekatsu;Wataru Marubashi;Wouter G. van Doorn;Tetsuya Yamada
在杂交烟草细胞中检测到具有空泡细胞死亡特征的关键信息PCD，包括与自噬相关的特征，并建立了空泡细胞死亡特征的详细时间历程。这种致死是由杂交幼苗的程序性细胞死亡(PCD)引起的，但这种PCD只发生在28℃下生长的幼苗和悬浮培养细胞中，而不发生在36℃下生长的植物PCD中。诱导自噬、空泡膜塌陷和肌动蛋白解体都是空泡细胞死亡的已知特征，但同时显示所有这些特征的PCD病例很少。在这项研究中，这些空泡细胞死亡的特征在表达杂交致死的杂交烟草细胞中是明显的。细胞离子渗漏、质膜断裂、空泡加工酶活性升高、空泡膜塌陷、点状F-肌动蛋白灶形成。透射电子显微镜显示，在这些细胞中形成了巨大的自噬结构，液泡膜破裂。在28℃的细胞死亡前，包含单甘酯(MDC)染色结构的细胞数量和9个自噬相关基因转录本的丰度增加；这些特征在36℃时不明显。我们评估了自噬抑制剂Wortmannin(WM)是否影响杂交细胞的致死率。在杂交细胞开始死亡后，西药抑制了离子泄漏和细胞死亡的增加，并减少了含有MDC染色结构的细胞数量。这些结果表明，在容易致死的杂交烟草细胞中，有几个明显的自噬和空泡细胞死亡的特征。此外，我们记录了这些空泡细胞死亡特征的详细时间过程。
Key messagePCD with features of vacuolar cell death including autophagy-related features were detected in hybrid tobacco cells, and detailed time course of features of vacuolar cell death were established.AbstractA type of interspecificNicotianahybrid,Nicotiana suaveolens×N. tabacumexhibits temperature-sensitive lethality. This lethality results from programmed cell death (PCD) in hybrid seedlings, but this PCD occurs only in seedlings and suspension-cultured cells grown at 28 °C, not those grown at 36 °C. Plant PCD can be classified as vacuolar cell death or necrotic cell death. Induction of autophagy, vacuolar membrane collapse and actin disorganization are each known features of vacuolar cell death, but observed cases of PCD showing all these features simultaneously are rare. In this study, these features of vacuolar cell death were evident in hybrid tobacco cells expressing hybrid lethality. Ion leakage, plasma membrane disruption, increased activity of vacuolar processing enzyme, vacuolar membrane collapse, and formation of punctate F-actin foci were each evident in these cells. Transmission electron microscopy revealed that macroautophagic structures formed and tonoplasts ruptured in these cells. The number of cells that contained monodansylcadaverine (MDC)-stained structures and the abundance of nineautophagy-relatedgene transcripts increased just before cell death at 28 °C; these features were not evident at 36 °C. We assessed whether an autophagic inhibitor, wortmannin (WM), influenced lethality in hybrid cells. After the hybrid cell began to die, WM suppressed increases in ion leakage and cell deaths, and it decreased the number of cells containing MDC-stained structures. These results showed that several features indicative of autophagy and vacuolar cell death were evident in the hybrid tobacco cells subject to lethality. In addition, we documented a detailed time course of these vacuolar cell death features.
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影响因子：
4.3
作者：
Yoshihito Shinozaki;Toshimitsu Tanaka;I. Ogiwara;M. Kanekatsu;W. G. van Doorn;Tetsuya Yamada
通讯作者：
Yoshihito Shinozaki;Toshimitsu Tanaka;I. Ogiwara;M. Kanekatsu;W. G. van Doorn;Tetsuya Yamada
影响因子：
4.9
作者：
Merkulova, Ekaterina A.;Guiboileau, Anne;Yoshimoto, Kohki
通讯作者：
Yoshimoto, Kohki
影响因子：
7.4
作者：
M. Mino;K. Maekawa;K. Ogawa;H. Yamagishi;M. Inoue
通讯作者：
M. Mino;K. Maekawa;K. Ogawa;H. Yamagishi;M. Inoue
DNA 甲基化抑制剂处理对烟草 (Nicotiana suaveolens x N. tabacum) 培养细胞生长的影响，克服了致死性
DOI：
--
发表时间：
2014
期刊：
影响因子：
--
作者：
入江佳;丸橋亘;金勝一樹;山田哲也
通讯作者：
山田哲也
DOI：
--
发表时间：
1978
期刊：
影响因子：
--
作者：
J. Palta;P. Li
通讯作者：
P. Li