Suppressors of ectopic UNC-5 growth cone steering identify eight genes involved in axon guidance in Caenorhabditis elegans
Suppressors of ectopic UNC-5 growth cone steering identify eight genes involved in axon guidance in Caenorhabditis elegans
复制标题
DOI：
10.1006/dbio.1997.8790
复制
发表时间：
1998-02-01
影响因子：
2.7
通讯作者：
Culotti, JG
中科院分区：
文献类型：
--
作者：
Colavita, A;Culotti, JG
UNC-5引导受体响应UNC-6/netrin通路提示，沿秀丽隐杆线虫表皮向背侧定向生长轴突。当在通常向腹侧或纵向延伸的触觉神经元中异位表达时，UNC-5能够以依赖unc -6的方式将轴突重新定向到背侧。这构成了基因筛选的基础，以确定其他突变，如unc-6突变，抑制unc-5诱导的生长锥引导。这些突变可能识别出unc-5引导先锋轴突所需的新成分。在本文中，我们描述了8个异位unc-5诱导生长锥转向所需的基因。其中4个突变鉴定出了之前已知的轴突引导基因unc-6、unc-40、unc-34和unc-44，另外4个突变鉴定出了新的基因unc-129、seu-2和seu-3。其中一些突变导致轴突引导缺陷，类似于在unc-5突变体中发现的缺陷。我们认为部分或全部这些基因可能在发育重要的unc-5信号通路中起作用。(C) 1998学术出版社。
The UNC-5 guidance receptor, in response to the UNC-6/netrin path cue, orients growing axons in a dorsal direction along the epidermis of Caenorhabditis elegans. When ectopically expressed in the touch neurons, which normally extend ventrally or longitudinally, UNC-5 is able to reorient their axons toward the dorsal side in an UNC-6-dependent manner. This forms the basis of a genetic screen to identify other mutations that, like unc-6 mutations, suppress unc-5-induced growth cone guidance. These mutations may identify new components required for pioneer axon guidance by unc-5. In this paper, we describe eight genes that are required for ectopic unc-5-induced growth cone steering. Mutations in four of these identify the previously known axon guidance genes unc-6, unc-40, unc-34, and unc-44 and mutations in four others identify the novel genes unc-129, seu-2, and seu-3. Several of these mutations cause axon guidance defects similar to those found in unc-5 mutants. We propose that some or all of these genes may function in a developmentally important unc-5 signaling pathway. (C) 1998 Academic Press.