Isolation of genes encoding the Neurospora vacuolar ATPase. Analysis of vma-1 encoding the 67-kDa subunit reveals homology to other ATPases.
Isolation of genes encoding the Neurospora vacuolar ATPase. Analysis of vma-1 encoding the 67-kDa subunit reveals homology to other ATPases.
复制标题
DOI：
10.1016/s0021-9258(18)68175-x
复制
发表时间：
1988-10
期刊：
影响因子：
--
通讯作者：
E. J. Bowman;Karen Tenney;B. Bowman
中科院分区：
文献类型：
--
作者：
E. J. Bowman;Karen Tenney;B. Bowman
粗糙脉孢菌的液泡膜含有一个H+转运ATP酶，该酶至少由三个亚基组成，分子量分别为70，000、60，000和15，000。编码最大亚基的基因组和cDNA克隆，似乎含有酶的活性位点，已被分离和测序。该亚基的基因称为vma-1，包含6个小内含子（60-131个碱基对），编码607个氨基酸的亲水蛋白质，Mr 67，121。在序列内是一个假定的核苷酸结合区，与该亚基含有ATP水解位点的提议一致。该67-kDa多肽与高等植物液泡ATP酶的类似多肽具有高度同源性（总体上62%相同残基，蛋白质中间84%相同残基）。空泡ATP酶与F0 F1 ATP酶相关的假设得到了脉孢菌空泡ATP酶的67-kDa亚基与F0 F1 ATP酶的α和β亚基之间相当大的同源性的发现的有力支持。
The vacuolar membrane of Neurospora crassa contains a H+-translocating ATPase composed of at least three subunits with approximate molecular weights of 70,000, 60,000, and 15,000. Both genomic and cDNA clones encoding the largest subunit, which appears to contain the active site of the enzyme, have been isolated and sequenced. The gene for this subunit, designated vma-1, contains six small introns (60-131 base pairs) and encodes a hydrophilic protein of 607 amino acids, Mr 67,121. Within the sequence is a putative nucleotide-binding region, consistent with the proposal that this subunit contains the site of ATP hydrolysis. This 67-kDa polypeptide shows high homology (62% identical residues overall and 84% in the middle of the protein) to the analogous polypeptide of a higher plant vacuolar ATPase. The hypothesis that the vacuolar ATPase is related to F0F1 ATPases is strongly supported by the finding of considerable homology between the 67-kDa subunit of the Neurospora vacuolar ATPase and both the alpha and beta subunits of F0F1 ATPases.