Design and Characterization of a Three-terminal Transcriptional Device Through Polymerase Per Second
Design and Characterization of a Three-terminal Transcriptional Device Through Polymerase Per Second
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DOI：
10.1109/tnb.2009.2028687
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发表时间：
2009-09-01
影响因子：
3.9
通讯作者：
Del Vecchio, Domitilla
中科院分区：
文献类型：
--
作者：
Varadarajan, Prasanna Amur;Del Vecchio, Domitilla
在本文中，我们提供了使用每秒聚合酶（PoPS）作为输入和输出的三端转录装置的计算机输入输出表征。该装置由噬菌体 lambda 开关转录电路的充分表征的部分组装而成。我们将电压和蛋白质浓度以及电流和 PoPS 进行类比，以证明三端转录装置的特性在质量上与双极结型晶体管 (BJT) 相似。特别是，当它出现在 BJT 中时，该器件可以调整为作为线性放大器或开关运行。当该器件作为线性放大器工作时，可以获得两倍的增益，这比 BJT 中获得的增益要小得多（其中可以达到 100 倍的放大增益）。这一事实表明，从自然转录系统中提取的部分可能自然地被设计为主要用于处理和存储信息，而不是放大信号。
In this paper, we provide an in silico input-output characterization of a three-terminal transcriptional device employing polymerase per second (PoPS) as input and output. The device is assembled from well-characterized parts of the bacteriophage lambda switch transcriptional circuit. We draw the analogy between voltage and protein concentration and between current and PoPS to demonstrate that the characteristics of the three-terminal transcriptional device are qualitatively similar to those of a bipolar junction transistor (BJT). In particular, as it occurs in a BJT, the device can be tuned to operate either as a linear amplifier or as a switch. When the device operates as a linear amplifier, gains of twofolds can be obtained, which are considerably smaller than those obtained in a BJT (in which 100-fold amplification gains can be reached). This fact suggests that the parts extracted from natural transcriptional systems may be naturally designed mostly to process and store information as opposed to amplify signals.