Selective destruction by formaldehyde fixation of an H-2Kb serological determinant involving lysine 89 without loss of T-cell reactivity
Selective destruction by formaldehyde fixation of an H-2Kb serological determinant involving lysine 89 without loss of T-cell reactivity
复制标题
通过甲醛固定选择性破坏涉及赖氨酸 89 的 H-2Kb 血清学决定簇，且不损失 T 细胞反应性
DOI：
10.1007/bf00375375
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发表时间：
2004
期刊：
影响因子：
3.2
通讯作者：
A. Schmitt
中科院分区：
文献类型：
--
作者：
C. Hua;C. Langlet;M. Buferne;A. Schmitt
在功能分析的准备中，对H-2Kb特异性单克隆抗体（mAb）与甲醛（FOR）固定的H-2b脾细胞或肿瘤细胞结合的研究显示，9种检测的mAb中有3种失去了与FOR固定细胞的反应性，而其他mAb的反应性通常不会降低。这些mAb对未处理的H-2Kbm突变体细胞和对FOR处理的H-2Kb细胞的反应性的比较表明，对于三种mAb，对后者的反应性的完全丧失可能是由FOR改变赖氨酸89的结果，其在突变体bm 3中被丙氨酸取代。H-2KPb特异性同种异体反应性多克隆或单克隆CTL，所有这些都保留了与bm 3靶细胞的反应性，也保留了与FO固定的H-2b细胞的反应性，如冷靶抑制研究所示。H-2Kb特异性CTL可能与H-2Kb的“构象”决定簇反应，这取决于H-2Kb分子的α1和α2结构域的完整性。结果与FOR治疗选择性影响α1结构域中的血清学决定簇而不影响构象型CTL决定簇一致。
In preparation for functional analyses, a study of the binding of H-2Kb-specific monoclonal antibodies (mAb) to formaldehyde (FOR)-fixed H-2b spleen or tumor cells revealed that three of nine mAb tested had lost reactivity with the FOR-fixed cells, whereas the reactivity of the other mAb generally did not diminish. Comparison of the reactivity of these mAb on untreated H-2Kbm mutant cells and on FOR-treated H-2Kb cells suggests that for three mAb the total loss of reactivity on the latter could be a consequence of the alteration by FOR of lysine 89, which is substituted by alanine in mutant bm3. H-2KPb-specific alloreactive polyclonal or monoclonal CTL, all of which had retained reactivity with bm3 target cells, had also retained reactivity with FOR-fixed H-2b cells as indicated by cold target inhibition studies. The H-2Kb-specific CTL were probably reactive with “conformational” determinants of H-2Kb, which are dependent on the integrity of both the α1 and the α2 domains of the H-2Kb molecule. Results are compatible with FOR treatment selectively affecting a serological determinant in the α1 domain without affecting conformational-type CTL determinants.