PHOTOACTIVATED GILVOCARCIN-V INDUCES DNA-PROTEIN CROSS-LINKING IN GENES FOR HUMAN RIBOSOMAL-RNA AND DIHYDROFOLATE-REDUCTASE
PHOTOACTIVATED GILVOCARCIN-V INDUCES DNA-PROTEIN CROSS-LINKING IN GENES FOR HUMAN RIBOSOMAL-RNA AND DIHYDROFOLATE-REDUCTASE
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DOI：
10.1111/j.1751-1097.1994.tb05095.x
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发表时间：
1994-09-01
影响因子：
3.3
通讯作者：
HANAWALT, PC
中科院分区：
文献类型：
--
作者：
MATSUMOTO, A;FUJIWARA, Y;HANAWALT, PC
利用改良的Southern杂交技术，在人成纤维细胞的rRNA和二氢叶酸还原酶基因的基因水平上分析了与光活化的gilvocarcin V的DNA相互作用的性质。既没有检测到链间DNA交联，也没有检测到RNA与DNA的连接。然而，我们始终观察到，在这两个基因延迟DNA带出现在剂量依赖性的方式暴露后，光活化gilvocarcin V。这些延迟带增强时，基因组DNA制备没有蛋白酶K处理，这表明参与蛋白质在这种DNA相互作用。因为这些条带在蛋白酶K处理后消失，所以光活化的gilvocarcin V可能诱导DNA-蛋白质交联。
The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking.