A Water-soluble Conjugated Polymer for Thiol Detection Based on "Turn-off" Effect
A Water-soluble Conjugated Polymer for Thiol Detection Based on "Turn-off" Effect
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DOI：
10.1002/cjoc.201500247
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发表时间：
2015-08
影响因子：
5.4
通讯作者：
Gaoshan Zeng;Jie Li;Haomei Liang;Yan Yuan;Xiang Li;Chao Yin;Zhen Yang;Quli Fan;Xiaomei Lu;Wei Huang
中科院分区：
文献类型：
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作者：
Gaoshan Zeng;Jie Li;Haomei Liang;Yan Yuan;Xiang Li;Chao Yin;Zhen Yang;Quli Fan;Xiaomei Lu;Wei Huang
研究了一种基于“关闭”效应的新型水溶性共轭聚合物（WSCP）巯基检测方法。该WSCP通过二硫键用聚（乙二醇）（PEG）进行修饰，以实现在水溶液中的良好溶解性（34 mg/mL）和高量子产率（0.47）。二硫键的断裂导致水溶性PEG链与共轭主链的分离，这将导致探针的水溶性显著降低，并导致荧光猝灭。组合的直观图像和荧光分光光度计进一步证实，降低的溶解度产生疏水缀合骨架的聚集。该探针的荧光强度与谷胱甘肽（GSH）在1-200 nmol·L-1范围内呈良好的线性关系，检测限为16 nmol·L-1。通过对多种生物分子的测试，证实了该WSCP探针是一种对巯基具有高选择性的良好传感材料。用该探针对Hela细胞内的硫醇类物质进行检测，结果显示该探针对细胞内硫醇类物质具有良好的检测效果。该探针具有良好的灵敏度和选择性，可进一步用于体内实验。总之，这种共轭聚合物探针弥补了小分子探针的不足，有助于开发基于共轭聚合物的新型探针。
We investigated a novel water-soluble conjugated polymer (WSCP) for thiol detection based on "turn-off" effect. This WSCP was modified with poly(ethylene glycol) (PEG) by disulfide linkages to achieve good solubility in aqueous solution (34 mg/mL) and high quantum yield (0.47). The separation of water-soluble PEG chains from the conjugated backbone induced by the cleavage of the disulfide linkages would lead to a significant decrease of the water solubility and a dramatical fluorescence quenching of the probe. The combined intuitive images and fluorescence spectrophotometer further confirmed that decreased solubility produced an aggregation of the hydrophobic conjugated backbone. The fluorescence intensity of the probe showed a good linear relationship with glutathione (GSH) (1–200 nmol·L−1), and the detection limit was 16 nmol·L−1. This WSCP probe was confirmed to be a good sensing material with high selectivity to thiols by testing various biological molecules. And this WSCP probe exhibited good detection effect to intracellular thiols by testing Hela cells. Considering the good sensitivity and selectivity, the probe could be further used in vivo. In conclusion, this conjugated polymer probe made up for the drawbacks of the micromolecue probes and contributed to the development of new probes based on conjugated polymers.