ACTIVE SODIUM AND CHLORIDE TRANSPORT IN THE SINGLE CELLED MARINE ALGA HALICYSTIS OVALIS
ACTIVE SODIUM AND CHLORIDE TRANSPORT IN THE SINGLE CELLED MARINE ALGA HALICYSTIS OVALIS
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DOI：
10.1111/j.1748-1716.1960.tb01922.x
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发表时间：
1960-01-01
期刊：
影响因子：
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通讯作者：
LEVEDAHL, BH
中科院分区：
文献类型：
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作者：
BLOUNT, RW;LEVEDAHL, BH
本文研究了卵圆藻（halicystis ovalis）巨大空心细胞中钠离子和氯离子在液泡溶液和环境溶液之间的主动转运。利用Ussingand Zerahn（1951）的短路技术，用同位素法测定了单个细胞的离子通量。用同心移液管将液泡液替换为sep水，并将液泡电位差短路至零。钠的平均净流出占短路电池电流的39.2% (s.d. = 5.4)，氯的平均净流入占57.6% （s.d. = 5.3）。因此，两个净有功磁通所携带的电流的总和可以解释总短路电流。微电极对细胞原生质的渗透表明，原生质的电位差与液泡电位差相同；总电位差在外膜处形成。提出了一种理论来评估这些运输系统对总电位差和细胞相对于环境的渗透稳定性的影响。
The active transports of sodium and chloride ions, between the vacuole and environmental solutions, were measured in the giant coenocyte of the marine algaHalicystis ovalis.Ion fluxes, determined isotopically, of individual single cells were measured by the short‐circuit technique of Ussingand Zerahn(1951). Concentric pipettes were used to replace the vacuole sap with sep water and to short‐circuit the vacuole potential difference to zero. The mean net efflux of sodium represented 39.2%, S. D. = 5.4, and the mean net influx of chloride 57.6%, S. D. = 5.3, of the current flowing through a short‐circuited cell. Therefore a summation of the current carried by the two net active fluxes can account for the total short‐circuit current. Micro‐electrode penetration of the protoplasm of the cell indicated that the potential difference of the protoplasm was identical with the vacuole potential; that the total potential difference develops at the outer membrane. A theory is presented which evaluates the effect of these transport systems on the total potential difference and on the osmotic stability of the cell relative to its environment.