Efficient Detection and Single-Cell Extraction of Circulating Tumor Cells in Peripheral Blood
Efficient Detection and Single-Cell Extraction of Circulating Tumor Cells in Peripheral Blood
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外周血中循环肿瘤细胞的高效检测和单细胞提取
DOI：
10.1021/acsabm.0c00957
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发表时间：
2020
影响因子：
4.7
通讯作者：
Guo Shi-shang
中科院分区：
文献类型：
--
作者：
Li Rui;Gong Zhiyi;Yi Kezhen;Li Wei;Liu Yichao;Wang Fubing;Guo Shi-shang
循环肿瘤细胞（CTC）在癌症生物学研究中发挥着重要作用。为了进一步阐明单个CTC在肿瘤转移和预后中的作用，必须开发有效的方法来分离和封装单个CTC。在这项工作中，构建了基于ZnO纳米纤维和表面声波的单一CTC捕获和封装平台。我们还证明了该平台可以有效地捕获和封装单个 CTC。我们首先验证了致密的 ZnO 纳米纤维提供了额外的结合位点，从而将捕获效率提高至高达 93.3%。然后我们证明，通过用超低浓度（25 mM）磷酸溶解底物，释放效率提高至100%，并且释放的细胞的活性不受影响。此外，释放的细胞悬浮在藻酸盐溶液中，并通过基于液滴的表面声波装置封装单个CTC。在520μs脉宽下，单个细胞的封装率高达13%。很少有基于纳米纤维和声学镊子通过声学液滴汽化进行单细胞封装的技术的报道。它在获取单个靶细胞方面具有广泛的潜在应用，这可能有助于进一步促进癌症患者的早期临床诊断和治疗。
Circulating tumor cells (CTCs) play an important role in cancer biology studies. To further elucidate the role of single CTCs in tumor metastasis and prognosis, effective methods must be developed to isolate and encapsulate single CTCs. In this work, a single CTC capture and encapsulation platform based on ZnO nanofibers and surface acoustic waves was constructed. We also demonstrated that this platform can capture and encapsulate single CTCs efficiently. We first validated that the dense ZnO nanofibers provide additional binding sites, resulting in an increased capture efficiency of up to 93.3%. We then demonstrated that the release efficiency was increased to 100% by dissolving the substrate with ultralow concentration (25 mM) phosphoric acid, and the activity of the released cells was not affected. Furthermore, the released cells were suspended in alginate solution and encapsulated single CTCs via droplet -based surface acoustic wave devices. The encapsulating rate of a single cell is up to 13% under a pulse width duration of 520 mu s. Few technology based on nanofibers and acoustic tweezers for single-cell encapsulation via acoustic droplet vaporization has been reported. It has a wide range of potential applications to acquire single target cells, which may facilitate further early clinical diagnosis and treatment of cancer patients.