Poly(ε-caprolactone) modification via surface initiated atom transfer radical polymerization with bio-inspired phosphorylcholine.
Poly(ε-caprolactone) modification via surface initiated atom transfer radical polymerization with bio-inspired phosphorylcholine.
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DOI：
10.1016/j.msec.2017.03.165
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发表时间：
2017-08
期刊：
影响因子：
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通讯作者：
Liu He;Lei Huang;Songbai Zhang;Yuan-wei Chen;Xianglin Luo
中科院分区：
文献类型：
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作者：
Liu He;Lei Huang;Songbai Zhang;Yuan-wei Chen;Xianglin Luo
材料表面的仿生磷胆碱修饰在构建生物相容性材料方面显示出巨大的应用前景。为了抑制蛋白质吸附和细胞黏附，改善血液相容性，本研究在聚己内酯(ε)表面生长了聚(2-甲基丙烯酰氧乙基)磷酰胆碱(PMPC)。将表面引发原子转移自由基聚合(SI-ATRP)的引发剂共价拴在PCL表面，然后将不同接枝量的PMPC刷接枝到PCL膜上。用X射线光电子能谱(XPS)和水接触角测量对改性后的片材进行了表征。与PCL膜相比，PMPC接枝的PCL膜对蛋白质的吸附较低，保持了分离蛋白的二级结构，抑制了血小板的黏附和伪足的形成，并保持了较长的活化部分凝血活酶时间(APTT)。同时，PMPC接枝的PCL膜抑制了LO2细胞的黏附。这些结果表明，在PCL表面进行磷胆碱SI-ATRP修饰可以使PCL在生物材料应用中具有更好的生物相容性。
Bio-inspired phosphorylcholine modification on material surface has shown great promise in constructing biocompatible materials. In this study, poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was grown on poly(ε-caprolactone) (PCL) surface in order to suppress protein adsorption and cells adhesion and to improve blood compatibility. The initiator for surface initiated atom transfer radical polymerization (SI-ATRP) was covalently tethered on PCL surface and then PMPC brushes with diverse graft amounts were grafted to PCL film. X-ray photoelectron spectroscopy (XPS) and water contact angle measurement were used to characterize the modified sheets. The PMPC-grafted PCL sheets showed lower protein adsorption, maintained secondary structure of detached protein, and suppressed adhesion and pseudopodium formation of the platelets, along with keeping longer activated partial thromboplastin time (APTT) in comparison with PCL membranes. At the same time, PMPC-grafted PCL sheets suppressed the LO2 cells adhesion. These results showed that phosphorylcholine SI-ATRP modification on PCL surface may provide PCL more biocompatible in biomaterial applications.