Phytosulfokine-α requires auxin to stimulate carrot non-embryogenic cell proliferation
Phytosulfokine-α requires auxin to stimulate carrot non-embryogenic cell proliferation
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DOI：
10.1016/s0981-9428(03)00052-4
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发表时间：
2003-05-01
影响因子：
6.5
通讯作者：
Kamada, H
中科院分区：
文献类型：
--
作者：
Eun, CH;Ko, SM;Kamada, H
植物硫因子-α(PSK-α)最初是从芦笋叶肉细胞培养的条件培养液中分离出来的一种多肽类植物生长因子，在悬浮培养中对某些植物的细胞增殖具有刺激作用。然而，植物激素和这种生长因子在植物中的生理关系还不是很清楚。利用胡萝卜(Daucus carota L.)在非胚性细胞培养中，我们研究了PSK-α对生长素[2，4-二氯苯氧乙酸(2，4-D)、吲哚-3-乙酸(IAA)或萘乙酸(NAA)]和PSK-α不同组合和浓度对细胞增殖的刺激作用。当生长素浓度较高时，PSK-α对细胞增殖无刺激作用，但在较低浓度时可促进细胞增殖。在没有生长素的情况下，细胞没有增殖。这些结果表明，当细胞分裂活性较低时，PSK-α刺激细胞增殖，这一过程需要生长素。我们还在竞争ELISA系统中评估了来自含高或低浓度生长素(2，4-D、IAA或NAA)的非胚性细胞培养的PSK-α的产生。高浓度生长素比低浓度生长素产生更多的PSK-α。这些结果表明生长素参与了PSK-α的产生。(C)2003年版《爱思唯尔科学与医学》。版权所有。
Phytosulfokine-alpha (PSK-alpha), a peptidyl plant growth factor that was originally isolated from conditioned medium derived from asparagus mesophyll cell culture, stimulates cell proliferation in some plants in suspension culture. However, the physiological relationship between phytohormones and this growth factor in plants is not well understood. Using carrot (Daucus carota L.) non-embryogenic cell culture, we investigated the stimulatory effects of PSK-alpha on the cell proliferation induced by different combinations and concentrations of auxin [2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), or naphthaleneacetic acid (NAA)] and PSK-alpha. Cell proliferation was not stimulated by PSK-alpha at high auxin concentrations, but was promoted at low auxin concentrations. There was no proliferation in the absence of auxin. These results indicate that PSK-alpha stimulates cell proliferation when cell division activity is low, and that auxin is required for this process. We also evaluated PSK-alpha production using medium derived from non-embryogenic cell culture with high or low concentrations of auxin (2,4-D, IAA or NAA) in a competition ELISA system. More PSK-alpha was produced at high auxin concentrations than at low auxin concentrations. These results suggest that auxin participates in PSK-alpha production. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.