Genotyping isolates of the entomopathogenic fungus Beauveria bassiana by RAPD with fluorescent labels
Genotyping isolates of the entomopathogenic fungus Beauveria bassiana by RAPD with fluorescent labels
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DOI：
10.1006/jipa.1997.4727
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发表时间：
1998-03-01
影响因子：
3.4
通讯作者：
Grau, O
中科院分区：
文献类型：
--
作者：
Berretta, MF;Lecuona, RE;Grau, O
采用随机扩增多态性DNA （RAPD）结合荧光脱氧核苷酸对阿根廷和巴西白僵菌分离株的遗传多样性进行了研究。在扩增产物的聚丙烯酰胺凝胶电泳过程中，通过自动激光荧光分析在线生成高分辨率的DNA指纹。每个分离物显示出不同的基因型。聚类分析显示，这些基因型之间存在高度变异。与地理来源或宿主没有相关性。然而，80%相似的表型组主要代表对甘蔗螟虫（Diatraea saccharalis）具有高毒力的分离株。基于荧光的RAPD指纹图谱为昆虫病原真菌的鉴定提供了一种有用的工具，该技术特别适用于种群研究中许多分离物的筛选。(C) 1998学术出版社。
Random amplified polymorphic DNA (RAPD) with incorporation of fluorescent deoxynucleotides was used to examine the genetic diversity among Beauveria bassiana isolates from Argentina and Brazil. High-resolution DNA fingerprints were generated on line, during polyacrylamide gel electrophoresis of amplification products, by automated laser fluorescence analysis. Each isolate displayed a distinct genotype. Cluster analysis showed a high level of variability among these genotypes. No correlation with geographical origin or host was detected. Nevertheless, a phenetic group of 80% similarity represented mainly the isolates exhibiting high virulence against the sugar cane borer, Diatraea saccharalis. Fluorescence-based RAPD fingerprints provide a useful tool for identifying entomopathogenic fungi, and this technique is specially applicable to screening many isolates in population studies. (C) 1998 Academic Press.