Diverse regulation of sensory signaling by C-elegans nPKC-epsilon/eta TTX-4
Diverse regulation of sensory signaling by C-elegans nPKC-epsilon/eta TTX-4
复制标题
DOI：
10.1038/sj.emboj.7600697
复制
发表时间：
2005-06-15
期刊：
影响因子：
11.4
通讯作者：
Mori, I
中科院分区：
文献类型：
--
作者：
Okochi, Y;Kimura, KD;Mori, I
体外分子和药理学研究表明，蛋白激酶C（PKC）家族成员在细胞内信号转导中起重要作用。然而，PKC在体内的作用知之甚少。在这里，我们表明，nPKC-ESTA TTX-4在线虫秀丽隐杆线虫是必需的各种感觉神经元的温度，气味，味道，和高渗透压的信号转导的调节。有趣的是，不同的感觉神经元对TTX-4的需求不同。在AFD热敏神经元中，TTX-4功能的获得或丧失分别使神经活动失活或过度活化，表明TTX-4对温度感觉的负调节。相反，TTX-4正调控ASH伤害性神经元的信号感觉。此外，在AWA和AWC嗅觉神经元中，TTX-4与另一个nPKC TPA-1一起发挥部分冗余的作用来调节嗅觉信号。这些结果表明C.秀丽线虫nPKC调节各种感觉神经元中不同的感觉信号传导。因此，C.秀丽隐杆线虫提供了一个理想的模型来揭示感觉信号中nPKC介导的分子途径的遗传新成分。
Molecular and pharmacological studies in vitro suggest that protein kinase C (PKC) family members play important roles in intracellular signal transduction. Nevertheless, the in vivo roles of PKC are poorly understood. We show here that nPKC-epsilon/eta TTX-4 in the nematode Caenorhabditis elegans is required for the regulation of signal transduction in various sensory neurons for temperature, odor, taste, and high osmolality. Interestingly, the requirement for TTX-4 differs in different sensory neurons. In AFD thermosensory neurons, gain or loss of TTX-4 function inactivates or hyperactivates the neural activity, respectively, suggesting negative regulation of temperature sensation by TTX-4. In contrast, TTX-4 positively regulates the signal sensation of ASH nociceptive neurons. Moreover, in AWA and AWC olfactory neurons, TTX-4 plays a partially redundant role with another nPKC, TPA-1, to regulate olfactory signaling. These results suggest that C. elegans nPKCs regulate different sensory signaling in various sensory neurons. Thus, C. elegans provides an ideal model to reveal genetically novel components of nPKC-mediated molecular pathways in sensory signaling.