Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31
Establishment of cell fate during early Drosophila embryogenesis requires transcriptional Mediator subunit dMED31
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DOI：
10.1016/j.ydbio.2007.11.019
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发表时间：
2008-01-15
影响因子：
2.7
通讯作者：
Sibon, Ody C. M.
中科院分区：
文献类型：
--
作者：
Bosveld, Floris;van Hoek, Sjoerd;Sibon, Ody C. M.
在早期果蝇胚胎发育过程中，前-后（A/P）轴的形成依赖于母体形态发生蛋白的空间梯度。众所周知，位置信息从这些形态发生素传递到差距基因。然而，这些信息是如何传播的，在很大程度上是未知的。转录介体复合物参与染色质状态、转录因子和RNA聚合酶II转录机制之间的信号传导的微调。我们发现，在保守的亚基的调解人复杂，dMED 31，阻碍胚胎发生前原肠胚形成，并导致异常表达的差距基因knirps和Kruppel和配对规则基因fusi tarazu和甚至跳过沿着A/P轴。在dMED 31突变体中，母体形态发生素背侧和驼背的表达不受影响。dMED 31的mRNA表达恰好在母体基因和差距基因的最高表达水平之间达到峰值。总之，我们的研究结果指出了dMED 31在指导母体形态基因指导的合子间隙基因表达中的作用，并提供了第一个体内证据，证明了介体复合物在黑腹果蝇细胞胚盘阶段建立细胞命运中的作用。(C)2007年爱思唯尔公司All rights reserved.
During early Drosophila embryogenesis, formation of the anterior-posterior (A/P) axis depends on spatial gradients of maternal morphogens. It is well recognized that positional information is transmitted from these morphogens to the gap genes. However, how this information is being transmitted is largely unknown. The transcriptional Mediator complex is involved in the fine tuning of the signaling between chromatin status, transcription factors and the RNA polymerase II transcription machinery. We found that a mutation in the conserved subunit of the Mediator complex, dMED31, hampers embryogenesis prior to gastrulation and leads to aberrant expression of the gap genes knirps and Kruppel and the pair-rule genes fusi tarazu and even-skipped along the A/P axis. Expression of the maternal morphogens dorsal and hunchback was not affected in dMED31 mutants. mRNA expression of dMED31 exactly peaks between the highest expression levels of the maternal genes and the gap genes. Together, our results point to a role for dMED31 in guiding maternal morphogen directed zygotic gap gene expression and provide the first in vivo evidence for a role of the Mediator complex in the establishment of cell fate during the cellular blastoderm stage of Drosophila melanogaster. (C) 2007 Elsevier Inc. All rights reserved.