Germline mutagenesis mediated by Sleeping Beauty transposon system in mice.
Germline mutagenesis mediated by Sleeping Beauty transposon system in mice.
复制标题
小鼠的睡美人转座系统介导的种系诱变。
DOI：
10.1186/gb-2007-8-s1-s14
复制
发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Horie, Kyoji
中科院分区：
文献类型：
--
作者：
Takeda, Junji;Keng, Vincent W;Horie, Kyoji
Following the descovery of its transposition activity in mammalian culture systems, the Sleeping Beauty (SB) transposon has since been applied to achieve germline mutagenesis in mice. Initially, the transposition efficiency was found to be low in cultured systems, but its activity in germ cells was unexpectedly high. This difference in transposition efficiency was found to be largely dependent on chromosomal status of the host genomic DNA and transposon vector design. The SB transposon system has been found to be suitable for comprehensive mutagenesis in mice. Therefore, it is an effective tool as a forward genetics screen for tagged insertional mutagenesis in mice.
