Head to tail organization of the human COL6A1 and COL6A2 genes by fiber-FISH.
Head to tail organization of the human COL6A1 and COL6A2 genes by fiber-FISH.
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通过纤维 FISH 对人类 COL6A1 和 COL6A2 基因进行头尾组织。
DOI：
10.1006/geno.1995.9008
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Chu,ML
中科院分区：
文献类型：
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作者：
Heiskanen,M;Saitta,B;Palotie,A;Chu,ML
两个VI型胶原基因，COL 6A 1和COL 6A 2，都映射到21q22.3，但这两个基因相对于彼此的顺序，距离和组织尚不清楚。最近开发的高分辨率荧光原位杂交（FISH）技术有很大的潜力，以促进精细分辨率的基因密度高的端粒区域的地图的建设。在此，我们使用Fiber-FISH确定了COL 6A 1和COL 6A 2基因之间的距离（150 kb）、COL 6A 1基因的大小（29 kb）以及这些基因的5′-3′方向（5′ COL 6A 1 3′-5′ COL 6A 2 3′）。
Two type VI collagen genes, COL6A1 and COL6A2, both map to 21q22.3, but the order, distance, and organization of these two genes relative to one another were not known. Recently developed high-resolution fluorescencein situhybridization (FISH) techniques have great potential to facilitate the construction of fine-resolution maps of telomeric regions where gene density is high. Here we have determined the distance separating the COL6A1 and COL6A2 genes (150 kb), the size of the COL6A1 gene (29 kb), and the 5′–3′ orientation of these genes (5′ COL6A1 3′–5′ COL6A2 3′) using fiber-FISH.