CULTURED ADHERENT CELLS FROM MARROW CAN SERVE AS LONG-LASTING PRECURSOR CELLS FOR BONE, CARTILAGE, AND LUNG IN IRRADIATED MICE
CULTURED ADHERENT CELLS FROM MARROW CAN SERVE AS LONG-LASTING PRECURSOR CELLS FOR BONE, CARTILAGE, AND LUNG IN IRRADIATED MICE
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DOI：
10.1073/pnas.92.11.4857
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发表时间：
1995-05-23
影响因子：
11.1
通讯作者：
PROCKOP, DJ
中科院分区：
文献类型：
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作者：
PEREIRA, RF;HALFORD, KW;PROCKOP, DJ
将来自表达人胶原蛋白I的微型基因的转基因小鼠的细胞用作标记物，以跟踪来自骨髓的间充质前体细胞的命运，所述骨髓通过粘附于塑料而部分富集，在培养物中扩增，然后注射到受辐射的小鼠中。标记物胶原蛋白I基因的灵敏PCR测定表明，在1周后，受体小鼠中存在很少的供体细胞，但在1-5个月后，除了骨髓和脾之外，供体细胞占骨、软骨和肺中细胞的1.5-12%。对肺的PCR原位测定表明，供体细胞弥散地分布在实质中，并且逆转录-PCR测定表明，标记胶原蛋白I基因以组织特异性方式表达。因此，证明了在培养中扩增的来自骨髓的间充质前体细胞可以作为骨、软骨和肺中间充质细胞的持久前体。他们表明细胞可能是离体基因治疗的特别有吸引力的靶点。
Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice, Sensitive PCR assays for the marker collagen I gene indicated that few of the donor cells were present in the recipient mice after 1 week, but 1-5 months later, the donor cells accounted for 1.5-12% of the cells in bone, cartilage, and lung in addition to marrow and spleen, A PCR in situ assay on lung indicated that the donor cells diffusely populated the parenchyma, and reverse transcription-PCR assays indicated that the marker collagen I gene was expressed in a tissue-specific manner, The results, therefore, demonstrated that mesenchymal precursor cells from marrow that are expanded in culture can serve as long-lasting precursors for mesenchymal cells in bone, cartilage, and lung, They suggest that cells may be particularly attractive targets for gene therapy ex vivo.