Improved surface plasmon resonance biosensing using silanized optical fibers
Improved surface plasmon resonance biosensing using silanized optical fibers
复制标题
DOI：
10.1016/j.snb.2015.04.069
复制
发表时间：
2015-09-01
影响因子：
8.4
通讯作者：
Lammertyn, Jeroen
中科院分区：
文献类型：
--
作者：
Arghir, Iulia;Spasic, Dragana;Lammertyn, Jeroen
表面等离子体共振 (SPR) 与光纤 (FO) 技术的耦合带来了该领域的巨大进步，与传统的基于棱镜的 SPR 平台相比，它具有诱人的优势，例如简单性、成本效益和小型化。然而，现有 FO-SPR 传感器的性能很大程度上取决于金 (Au) 层与 FO 二氧化硅核心的粘附力，因此通常是实现基准 SPR 系统性能的主要限制因素。在本文中，我们使用（3-羧基丙基）三甲氧基硅烷（MPTMS）作为附着力促进剂，在三维（3D）FO-SPR 传感探针上开发坚固的金表面。精心制备的 FO 基底首先使用湿化学方法进行硅烷化，MPTMS 浓度范围为 2.5 至 24 mM，随后在室温 (RT) 或 100 摄氏度下进行干燥处理，然后涂上类似于 50 nm Au 等离子体薄膜。接下来，通过在蔗糖稀释液中进行折射率 (RI) 测量，使用不同制备的硅烷化 FO 来评估其灵敏度。对获得的数据进行高级统计分析表明，使用 8 mM MPTMS 溶液结合 RT 后干燥处理是生产具有显着改善的 Au 粘附性能的 FO 的有效方法。通过将参考和硅烷化 FO 暴露在应力条件下，例如强机械（胶带测试）、化学（食人鱼溶液处理）和热变化，进一步研究了 MPTMS 底层的作用。尽管使用扫描电子显微镜 (SEM) 的其他研究揭示了这些耐久性测试后金膜形态的变化，但硅烷化 FO 表现出增强的稳健性，同时保留了传感器的整体功能。相比之下，参考 FO 始终未能通过机械和化学测试，而仅能抵抗热变化。此外，硅烷化 FO-SPR 探针的耐受性得到改善，可重复使用多达 3 次，而传感器性能不会明显损失，同时基于两种类型的生物受体（针对凝血酶蛋白的 DNA 适体和针对人免疫球蛋白 E hIgE 的多克隆抗体）进行生物测定。所有这些结果可能代表着在制造更强大和可重复使用的 FO-SPR 生物传感器方面向前迈出了一步，具有开发高灵敏度生化测定的巨大潜力。 (C) 2015 Elsevier B.V. 保留所有权利。
Coupling surface plasmon resonance (SPR) to optical fiber (FO) technology has brought tremendous advancements in the field by offering attractive advantages over the traditional prism-based SPR platforms, such as simplicity, cost-effectiveness and miniaturization. However, the performance of the existing FO-SPR sensors widely depends on the adhesion of the gold (Au) layer to the FO silica core, thereby often representing a major limiting factor in achieving the properties of the benchmark SPR systems. In this paper, we used (3-marcaptopropyl)trimethoxysilane (MPTMS) as an adhesion promoter for developing robust Au surfaces on the three-dimensional (3D) FO-SPR sensing probe. Carefully prepared FO substrates were first silanized using a wet chemistry approach, with MPTMS concentrations ranging from 2.5 to 24 mM, and subsequently exposed to a drying treatment at room temperature (RT) or at 100 degrees C, before coating them with a similar to 50 nm Au plasmonic film. Differently prepared silanized FOs were next used for evaluating their sensitivities, by performing refractive index (RI) measurements in sucrose dilutions. Advanced statistical analysis of the obtained data indicated that using 8 mM MPTMS solution coupled with a RT post-drying treatment is an efficient way of producing FOs with dramatically improved Au adhesion properties. The role of the MPTMS underlayer was further investigated by exposing the reference and silanized FOs to stress conditions, such as strong mechanical (adhesion tape tests), chemical (piranha solution treatments) and thermal variations. Although additional studies using scanning electron microscopy (SEM) revealed changes in the Au film morphology after these endurance tests, the silanized FOs exhibited an enhanced robustness while retaining the overall sensor's capabilities. In contrast, the reference FOs consistently failed the mechanical and chemical tests, while only resisting under thermal variations. Moreover, the improved resistance of the silanized FO-SPR probes allowed them to be reused up to three times with no significant loss in the sensor performance, while implementing bioassays based on two types of bioreceptors (a DNA aptamer against thrombin protein and a polyclonal antibody against human immunoglobulin E hIgE). All these results might represent a step forward in the fabrication of more robust and reusable FO-SPR biosensors, featuring great potential for developing highly-sensitive biochemical assays. (C) 2015 Elsevier B.V. All rights reserved.