Enhancement of Cortical Network Activity in vitro and Promotion of GABAergic Neurogenesis by Stimulation with an Electromagnetic Field with a 150 MHz Carrier Wave Pulsed with an Alternating 10 and 16 Hz Modulation.
Enhancement of Cortical Network Activity in vitro and Promotion of GABAergic Neurogenesis by Stimulation with an Electromagnetic Field with a 150 MHz Carrier Wave Pulsed with an Alternating 10 and 16 Hz Modulation.
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DOI：
10.3389/fneur.2015.00158
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发表时间：
2015
影响因子：
3.4
通讯作者：
Pries W
中科院分区：
文献类型：
--
作者：
Gramowski-Voß A;Schwertle HJ;Pielka AM;Schultz L;Steder A;Jügelt K;Axmann J;Pries W
近年来，各种刺激被确定能够增强神经发生，这是一个在衰老的大脑和神经退行性疾病和某些神经精神疾病中功能失调的过程。电磁场在脑组织中的应用已被证明会影响细胞特性，并且它们在医学治疗中的重要性已得到认可。在这项研究中，区分小鼠皮层网络的多孔微电极阵列反复暴露于极低的电磁场（ELEMF）与交替的10和16 Hz的频率背驮到150 MHz的载波频率。ELEMF暴露刺激了电网络活动，强化了爆发的结构。此外，在网络活动分化的前28天内暴露于电磁场也诱导了爆发结构内的重组。这种效应在体外暴露10天后的14天时已经最明显。总的来说，在这些条件下，皮层活动的发展加速了。这些功能性的电生理变化伴随着形态学的。神经元胶质共培养物中神经元的百分比增加而不影响细胞的总数，表明神经发生的增强。ELEMF暴露选择性地促进特定神经元群体的增殖，这由GABA能神经元的比例增加证明。结果支持了最初的假设，即这种ELEMF刺激可能是特定适应症的治疗选择，具有CNS应用的前景，特别是退行性疾病，如阿尔茨海默病和其他痴呆症。
In recent years, various stimuli were identified capable of enhancing neurogenesis, a process which is dysfunctional in the senescent brain and in neurodegenerative and certain neuropsychiatric diseases. Applications of electromagnetic fields to brain tissue have been shown to affect cellular properties and their importance for therapies in medicine is recognized. In this study, differentiating murine cortical networks on multiwell microelectrode arrays were repeatedly exposed to an extremely low-electromagnetic field (ELEMF) with alternating 10 and 16 Hz frequencies piggy backed onto a 150 MHz carrier frequency. The ELEMF exposure stimulated the electrical network activity and intensified the structure of bursts. Further, the exposure to electromagnetic fields within the first 28 days in vitro of the differentiation of the network activity induced also reorganization within the burst structure. This effect was already most pronounced at 14 days in vitro after 10 days of exposure. Overall, the development of cortical activity under these conditions was accelerated. These functional electrophysiological changes were accompanied by morphological ones. The percentage of neurons in the neuron glia co-culture was increased without affecting the total number of cells, indicating an enhancement of neurogenesis. The ELEMF exposure selectively promoted the proliferation of a particular population of neurons, evidenced by the increased proportion of GABAergic neurons. The results support the initial hypothesis that this kind of ELEMF stimulation could be a treatment option for specific indications with promising potential for CNS applications, especially for degenerative diseases, such as Alzheimer’s disease and other dementias.