Correction to: Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus.
Correction to: Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus.
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更正：生长刺激期间肌肉干细胞的分裂独立分化。
DOI：
10.1093/stmcls/sxae021
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发表时间：
2024
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众所周知，成体肌肉干细胞(MuSCs)在分化和融合之前会在激活时复制，以再生肌纤维。目前尚不清楚骨髓间充质干细胞分化是否与细胞分裂有内在联系，细胞分裂对干细胞种群的维持有影响。我们使用单细胞RNA测序来鉴定成年肌肉在生长刺激5天后在转录上不同的MuSCs亚群。轨迹推断与追踪MUSC来源的肌核的新小鼠模型和体内DNA复制标记相结合，揭示了MUSC群体表现出独立于分裂的分化和融合。这些发现表明，在存在完整的肌纤维的情况下，对生长刺激的反应，MUSC的分裂不是强制性的。
Adult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.