Chemoattraction enrichment of thymus-homing bone marrow cells.
Chemoattraction enrichment of thymus-homing bone marrow cells.
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胸腺归巢骨髓细胞的化学吸引富集。
DOI：
10.1111/j.1365-2184.1987.tb01101.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Taubenberger,JK
中科院分区：
文献类型：
--
作者：
Haar,JL;Grogan,WM;Popp,JD;Taubenberger,JK
据报道，小鼠骨髓中的一群细胞迁移到由新生小鼠胸腺碎片孵育而成的培养上清液中，并且迁移的群体中富含未成熟的淋巴样细胞。在目前的研究中，我们表明，这种方法丰富了胸腺归巢细胞。用异硫氰酸荧光素(FITC)标记富迁移细胞，并将其注射到致死照射小鼠的尾静脉中。实验组小鼠胸腺细胞悬液中有8.1±1.8%的荧光细胞，而对照组小鼠胸腺细胞悬液中有2.4±1.7%的阳性细胞，而对照组小鼠胸腺细胞悬液中有相同数量的非迁移富集型FITC标记细胞。
It has been reported that a population of cells from mouse bone marrow migrates to supernatant made from the incubation of minced fragments of new‐born mouse thymuses and that the migrated population is enriched for immature lymphoid cells. In the present study, we show that this method enriches for thymic‐homing cells. Migration‐enriched cells were labelled with fluorescein isothiocyanate (FITC) and were injected into the tail veins of lethally irradiated mice. Cell suspensions of the thymuses from these experimental mice had 8.1 ± 1.8% fluorescing cells compared to control mice given equal numbers of non‐migration‐enriched FITC labelled cells which had 2.4 ± 1.7% positive cells.