IMMUNE REACTIVITY TO DAR-P-I AND DER-P-II IN HOUSE-DUST-MITE SENSITIVE PATIENTS ATTENDING PEDIATRIC AND ADULT ALLERGY-CLINICS
IMMUNE REACTIVITY TO DAR-P-I AND DER-P-II IN HOUSE-DUST-MITE SENSITIVE PATIENTS ATTENDING PEDIATRIC AND ADULT ALLERGY-CLINICS
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DOI：
10.1111/j.1365-2222.1994.tb00984.x
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发表时间：
1994-08-01
影响因子：
6.1
通讯作者：
THOMAS, WR
中科院分区：
文献类型：
--
作者：
OBRIEN, RM;THOMAS, WR
比较了儿童和成人过敏门诊有症状患者对屋尘螨过敏原的免疫应答。IgE特异性免疫反应性屋尘螨进行了检查，通过Western印迹法和Der p I和Der p II，通过放射免疫斑点印迹法使用纯化的过敏原。比较了19名螨敏感儿童（平均年龄9岁）和26名成人（平均年龄31岁）。阳性IgE反应的斑点印迹法被发现在79%的儿童Der p I和Der p II，而反应性仅分别存在于23%和19%的成人，和光密度指示较弱的反应。在儿童中，Western印迹法表明，大部分血清学反应性针对Der p II（17/19，89%）或100 kDa组分，而在成人中，反应性通常针对其他组分，只有15/26（58%）识别Der p II。与其他人的结果一致，尽管斑点印迹呈阳性，但在Western分析中检测不到对应于Der p I的部分。为了确定成人中IgE血清学免疫反应性的相对缺乏是否与细胞识别的类似缺乏相关，也使用纯化的过敏原进行了T细胞增殖研究。有趣的是，这些结果显示，29%的受试者对Der p I和50%的受试者对Der p II存在细胞反应性（无血清学反应性）。在所有具有特异性IgE的个体中均出现明显的免疫应答。
A comparison was made of immune responses to house dust mite allergens in symptomatic patients from paediatric and adult allergy clinics. IgE-specific immune reactivity to Dermatophagoides pteronyssinus was examined by Western blotting and, for Der p I and Der p II, by radioimmune dot-blot using purified allergens. Nineteen mite-sensitive children (mean age 9 years) and 26 adults (mean age 31 years) were compared. Positive IgE responses by dot-blot were found to Der p I and Der p II in 79% of children, whereas reactivity was only present in 23% and 19% respectively of adults, and densitometry indicated a weaker response. In children, Western blotting indicated that the majority of the serologic reactivity was directed to Der p II (17/19, 89%) or a 100 kDa fraction whereas in adults, reactivities were generally directed to other fractions with only 15/26 (58%) recognizing Der p II. Consistent with results of others, a fraction corresponding to Der p I was poorly detected on Western analysis despite positive dot-blots. To determine whether the relative lack of IgE serological immune reactivity in adults was associated with a similar lack of cellular recognition, T-cell proliferation studies were performed, also using purified allergens. Interestingly, these revealed that cellular responsiveness, without serological reactivity, was present in 29% of subjects to Der p I and 50% to Der p II. Proliferative responses were evident in all individuals with specific IgE.