Adipose tissue-derived tumor necrosis factor-alpha activity is elevated in older rats
Adipose tissue-derived tumor necrosis factor-alpha activity is elevated in older rats
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DOI：
10.1093/gerona/52a.4.b190
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发表时间：
1997-07-01
影响因子：
5.1
通讯作者：
Eckel, RH
中科院分区：
文献类型：
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作者：
Morin, CL;Pagliassotti, MJ;Eckel, RH
高水平的脂肪组织来源的肿瘤坏死因子-α (AT-TNF) mRNA 和蛋白质先前已被认为与肥胖和胰岛素抵抗的一般模型有关。由于存在内源性 TNF 抑制剂，因此尚不清楚 AT-TNF 活性是否也会增加。我们推测，由于脂肪量的积累，老年动物的 AT-TNF 活性会增加。我们选择研究 2 名不同年龄的男性 Fischer 344 率，即 3 个月大（年轻）和 14 个月大（成熟），因为脂肪量应该有很大不同，但胰岛素对葡萄糖代谢的作用相似。事实上，成熟大鼠的脂肪量是其 1.5 倍以上，但根据空腹血浆胰岛素估计，全身胰岛素抵抗与年轻大鼠相似。成年大鼠附睾 (EPI) 和腹膜后 (Retro) 脂肪垫的 AT-TNF 活性是年轻大鼠的两倍 (p
High levels of adipose tissue-derived tumor necrosis factor-alpha (AT-TNF) mRNA and protein have previously been associated with generic models of obesity and insulin resistance. Because there are endogenous TNF inhibitors it is unknown if AT-TNF activity is also increased. We hypothesized that AT-TNF activity would increase in older ani mars because of an accumulation of fat mass. We chose to study 2 different-aged male Fischer 344 rate, 3-month-old (young) and 14-month-old (mature) because fat mass should be quite different but insulin action on glucose metabolism similar. Indeed, mature rats had over 1.5-fold more fat mass, but whole body insulin resistance, as estimated by fasting plasma insulin, was similar to young rats. Mature rats had twice as much AT-TNF activity as the young in both the epididymal (EPI) and retroperitoneal (Retro) fat pads (p