Subunit interactions during cooperative opening of voltage-gated proton channels.
Subunit interactions during cooperative opening of voltage-gated proton channels.
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DOI：
10.1016/j.neuron.2012.12.021
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发表时间：
2013-01-23
期刊：
影响因子：
16.2
通讯作者：
Larsson HP
中科院分区：
文献类型：
--
作者：
Qiu F;Rebolledo S;Gonzalez C;Larsson HP
电压门控质子（Hv1）通道是二聚体，其中每个亚基具有单独的渗透途径。然而，这两种途径的开放是高度合作的。目前尚不清楚Hv1通道如何打开其渗透途径，因为Hv1通道缺乏一个经典的孔域。使用电压钳荧光法，我们在这里检测两个构象变化报告的荧光团连接到电压传感器S4的Hv1通道。第一个是电压依赖性和通道开放之前，与独立的S4电荷运动在两个亚基的报告一致的属性。第二种是电压依赖性较小，与通道开放密切相关。减少二聚化或改变亚基间界面的突变影响第二构象变化和通道开放。这些观察结果表明，在两个亚基中的初始S4电荷运动之后，有第二个合作构象变化，涉及亚基之间的相互作用，打开Hv1通道中的两个通路。
Voltage-gated proton (Hv1) channels are dimers, where each subunit has a separate permeation pathway. However, opening of the two pathways is highly cooperative. It is unclear how Hv1 channels open their permeation pathways, because Hv1 channels lack a classic pore domain. Using voltage clamp fluorometry, we here detect two conformational changes reported by a fluorophore attached to the voltage sensor S4 in Hv1 channels. The first is voltage dependent and precedes channel opening, with properties consistent with reporting on independent S4 charge movements in the two subunits. The second is less voltage dependent and closely correlates with channel opening. Mutations that reduce dimerization or alter the intersubunit interface affect both the second conformational change and channel opening. These observations suggest that, following an initial S4 charge movement in the two subunits, there is a second, cooperative conformational change, involving interactions between subunits, that opens both pathways in Hv1 channels.
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影响因子：
25
作者：
通讯作者：
--
DOI：
10.1085/jgp.200910379
发表时间：
2010-07
期刊：
The Journal of general physiology
影响因子：
--
作者：
Iovannisci D;Illek B;Fischer H
通讯作者：
Fischer H
影响因子：
16.8
作者：
通讯作者：
--
振动钾通道门控。 II：激活途径中的过渡。
DOI：
10.1085/jgp.103.2.279
发表时间：
1994-02
期刊：
The Journal of general physiology
影响因子：
--
作者：
Zagotta WN;Hoshi T;Dittman J;Aldrich RW
通讯作者：
Aldrich RW
影响因子：
16.2
作者：
Hong L;Pathak MM;Kim IH;Ta D;Tombola F
通讯作者：
Tombola F