The DNA sequence of medaka chromosome LG22
The DNA sequence of medaka chromosome LG22
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DOI：
10.1016/j.ygeno.2006.09.003
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发表时间：
2007-01-01
期刊：
影响因子：
4.4
通讯作者：
Shimizu, Nobuyoshi
中科院分区：
文献类型：
--
作者：
Sasaki, Takashi;Shimizu, Atsushi;Shimizu, Nobuyoshi
我们报告了小型硬骨鱼青鳉（Oryzias latipes）单条染色体（连锁群 22；LG22）的基因组 DNA 序列，这是非哺乳动物脊椎动物的第一个完整染色体序列。从18,803,338 bp的DNA序列中推导出633个蛋白质编码基因的顺序和方向，为通过与人类基因组直接比较来分析脊椎动物基因组的染色体进化提供了机会。青鳉和人类之间“保守基因簇”（CGC）（序列基础上“同线性”的严格定义）中的平均基因数量为 1.6。这些数据和其他数据表明，在人类和青鳉谱系中，大约 38.8% 的成对基因关系可能是从它们的共同祖先中断裂的，并进一步表明，共同祖先的整个基因组中可能发生了大约 20,000 (15,520-23,280) 个断裂。这些断裂主要是由脊椎动物谱系特定时代的染色体内改组产生的。这些精确的比较基因组学使我们能够识别共同脊椎动物祖先的古代染色体片段，并估计脊椎动物谱系中的染色体进化。 (c) 2006 Elsevier Inc. 保留所有权利。
We report the genomic DNA sequence of a single chromosome (linkage group 22; LG22) of the small teleost fish medaka (Oryzias latipes) as a first whole chromosome sequence from a non-mammalian vertebrate. The order and orientation of 633 protein-coding genes were deduced from 18,803,338 bp of DNA sequence, providing the opportunity to analyze chromosome evolution of vertebrate genomes by direct comparison with the human genome. The average number of genes in the "conserved gene cluster" (CGC), a strict definition of "synteny" at the sequence basis, between medaka and human was 1.6. These and other data suggest that approximately 38.8% of pair-wise gene relationships would have been broken from their common ancestor in the human and medaka lineages and further imply that approx 20,000 (15,520-23,280) breaks would have occurred from the entire genome of the common ancestor. These breaks were generated mainly by intra-chromosomal shufflings at a specific era in the vertebrate lineage. These precise comparative genomics allowed us to identify the pieces of ancient chromosomes of the common vertebrate ancestor and estimate chromosomal evolution in the vertebrate lineage. (c) 2006 Elsevier Inc. All rights reserved.