Barium yttrium fluoride based upconversion nanoparticles as dual mode image contrast agents.

Barium yttrium fluoride based upconversion nanoparticles as dual mode image contrast agents.
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DOI：
10.1016/j.msec.2021.111937
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发表时间：
2021-02
期刊：
Materials science & engineering. C, Materials for biological applications
影响因子：
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通讯作者：
P. P. Nampi-P.;A. Vakurov;H. Viswambharan;J. Schneider;R. Brydson;P. Millner;S. Saha;G. Jose
P. P. Nampi-P.;A. Vakurov;H. Viswambharan;J. Schneider;R. Brydson;P. Millner;S. Saha;G. Jose
中科院分区：
其他
文献类型：
--
作者：
P. P. Nampi-P.;A. Vakurov;H. Viswambharan;J. Schneider;R. Brydson;P. Millner;S. Saha;G. Jose

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双标记造影剂可以提供更好的互补信息的生物成像比可单独从一个单一的模态。本文研究了Yb 3+和Er 3+掺杂的BaYF 5上转换纳米粒子（UCNPs）作为光学和X射线微计算机断层扫描（μCT）造影剂的适用性。采用水热法合成了稳定的UCNP水分散体，并加入了聚乙烯亚胺（PEI）。UCNP为单晶，具有截短的立方体和/或截短的八面体形态，透射电子显微镜的平均粒径为47 ±9 nm，透射电子显微镜进一步用于详细表征结构和组成。对于水性纳米颗粒分散体测量到+51mV的ζ电位值，这对于细胞渗透性是有益的。外部水合PEI层也有利于蛋白质的附着，用于生物系统中的靶向递送。当使用MTT测定法进行评估时，所制备的UCNP被证明在高达3.5 mg/mL的浓度下对内皮细胞无毒。当使用二极管激光器在976 nm的波长下激发时，颗粒显示出强烈的绿色上转换光致发光。定量X射线μCT造影成像证实了这些UCNP作为X射线造影剂的潜力，并证实了它们用于生物成像的双重模式。
Dual labeled contrast agents could provide better complementary information for bioimaging than available solely from a single modality. In this paper we investigate the suitability of Yb3+and Er3+-doped BaYF5upconversion nanoparticles (UCNPs) as both optical and X-ray micro computed tomography (μCT) contrast agents. Stable, aqueous UCNP dispersions were synthesised using a hydrothermal method with the addition of polyethyleneimine (PEI). UCNPs were single crystal and had a truncated cuboidal and/or truncated octahedral morphology, with average particle size of 47 ±9 nm from transmission electron microscopy which was further used to characterize the structure and composition in detail. A zeta potential value of +51 mV was measured for the aqueous nanoparticle dispersions which is beneficial for cell permeability. The outer hydrated PEI layer is also advantageous for the attachment of proteins for targeted delivery in biological systems. The prepared UCNPs were proven to be non-toxic to endothelial cells up to a concentration of 3.5 mg/mL, when assessed using an MTT assay. The particles showed intense green upconversion photoluminescence when excited at a wavelength of 976 nm using a diode laser. Quantitative X-ray μCT contrast imaging confirmed the potential of these UCNPs as X-ray contrast agents and confirming their dual modality for bioimaging.