The stromelysin-1 5A/5A genotype enhances colorectal cancer cell invasion in Iranian population
The stromelysin-1 5A/5A genotype enhances colorectal cancer cell invasion in Iranian population
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发表时间：
2012-10
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通讯作者：
M. Motovali-bashi;Z. Hojati;S. Hajihoseiny;S. Hemmati
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作者：
M. Motovali-bashi;Z. Hojati;S. Hajihoseiny;S. Hemmati
背景：基质金属蛋白酶是一类降解细胞外基质的酶。在一些基因的启动子区域存在单核苷酸多态性，这些基因能够影响癌症易感性。本研究旨在分析MMP 3基因启动子多态性与结直肠癌发生、发展的关系。材料与方法：在这项病例对照研究中，120例结直肠癌患者和100名对照使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的基因组脱氧核糖核酸（DNA）的基因分型。患者组分为不同的亚组：无转移活性的亚组（M-）和已发生转移的亚组（M+）。结果：MMP-3基因型频率在病例组和对照组间差异有显著性（χ 2 = 16.17; P = 0.0003）。5A纯合子在患者组和对照组之间有显著性差异。5A等位基因频率（67.91%）显著高于健康对照组（49%）（χ2 = 16.17，P = 0.00005）。在确诊时，携带5A等位基因的个体在M+亚组中的代表性高于M-亚组（χ 2 = 7.49; P = 0.006，OR = 3.86; 95%CI，1.43-10.33）。M-和对照组之间的差异没有观察到统计学显著性（χ² = 0.009; P = 0.92）。结论：我们的研究结果表明，在MMP-3启动子区域的5A多态性的存在可能有利于结直肠癌患者的生长和转移过程中，可以看作是一个危险因素，预后不良。
Background: Matrix metalloproteinases comprise a family of enzyme degrade components of extra cellular matrix. There are single nucleotide polymorphisms in the promoter regions of several genes with ability to influence cancer susceptibility. The aim of this study was to analyze association between MMP3 promoter polymorphisms and colorectal cancer occurrence and progression. Materials and Methods: In this case-control study 120 colorectal cancer patients and 100 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on the genomic deoxyribonucleic acid (DNA). The patients group was divided into different subgroups: a subgroup without metastatic activity (M-) and a subgroup that had developed metastasis (M+). Results: There was a significant difference in frequency of the MMP-3 genotype between cases and controls (χ = 16.17; P = 0.0003). The 5A homozygote in patients and controls was significantly different. The frequency of the 5A allele among affected patients (67.91%) was significantly higher than among the healthy controls (49%; χ2 = 16.17, P = 0.00005). At the time of diagnosis, individual who was carrying the 5A allele was more represented in the M+ subgroup than in M- subgroup (χ² = 7.49; P = 0.006, OR = 3.86; 95% CI, 1.43–10.33). The difference between M- and controls did not observe statistically significant (χ² = 0.009; P = 0.92). Conclusions: Our results suggest that the presence of 5A polymorphism at the MMP-3 promoter region may favor the growth and the metastasis process in colorectal cancer patients and could be looked at as a risk factor for a worse prognosis.