Development of a rapid 21-plex autosomal STR typing system for forensic applications
Development of a rapid 21-plex autosomal STR typing system for forensic applications
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开发用于法医应用的快速 21 重常染色体 STR 分型系统。
DOI：
10.1002/elps.201500498
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发表时间：
2016-10-01
期刊：
影响因子：
2.9
通讯作者：
Yan, Jiangwei
中科院分区：
文献类型：
--
作者：
Yang, Meng;Yin, Caiyong;Yan, Jiangwei
DNA-STR基因分型技术已广泛应用于法医学领域。即使取得了这样的成功，也非常需要减少分析时间。本研究建立了一种新的21重STR快速分型系统，包括Penta D、Penta E、D12 S391、D2 S1338、D 6S 1043、D19 S433、D2 S441和Amelogenin等13个CODIS位点。该系统可以将扩增时间缩短到最少90 min，并且不需要从样品中提取DNA。分型系统的验证符合DNA分析方法科学工作组（SWGDAM）和中国国家标准（GA/T815-2009）指南。结果表明，该21重STR分型系统是一个有价值的工具，快速刑事调查。
DNA-STR genotyping technology has been widely used in forensic investigations. Even with such success, there is a great need to reduce the analysis time. In this study, we established a new rapid 21-plex STR typing system, including 13 CODIS loci, Penta D, Penta E, D12S391, D2S1338, D6S1043, D19S433, D2S441 and Amelogenin loci. This system could shorten the amplification time to a minimum of 90 min and does not require DNA extraction from the samples. Validation of the typing system complied with the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Chinese National Standard (GA/T815-2009) guidelines. The results demonstrated that this 21-plex STR typing system was a valuable tool for rapid criminal investigation.