Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature
Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature
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DOI：
10.1007/s12374-017-0269-7
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发表时间：
2018-02
影响因子：
2.9
通讯作者：
Guoliang Yuan;Yukun Wang;Shao-hua Yuan;Peng Wang;Wen-jing Duan;Jian-fang Bai;Hui Sun;Na Wang;Feng-ting Zhang;Liping Zhang;Changping Zhao
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文献类型：
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作者：
Guoliang Yuan;Yukun Wang;Shao-hua Yuan;Peng Wang;Wen-jing Duan;Jian-fang Bai;Hui Sun;Na Wang;Feng-ting Zhang;Liping Zhang;Changping Zhao
温敏雄性不育在小麦育性和生产上具有重要作用。脱镁叶绿酸加氧酶（PaO）作为叶绿素降解的关键酶，可以抑制植物细胞死亡。从温敏核不育系BS 366中克隆了TaPaO 1基因，该基因编码一个典型的PaO蛋白，包含一个保守的Rieske [2Fe-2S]铁硫基序、一个单核非血红素铁结合基序和一个C端CxxC基序，在低温下在BS 366花药的所有组织中表达，并在减数分裂期表达上调。TaPaO 1的亚细胞定位特异性地标记了叶绿体周围，在转基因烟草中，RD 29 A启动子调控的TaPaO 1对低温有反应，导致花粉败育。表达分析表明，在低温处理过程中，TaPaO 1在转基因烟草花药中的表达量高于其它组织。推测低温条件下，TaPaO 1的高衰老相关活性可能导致花药发育早期的细胞死亡。这些结果为了解PaO在花药发育早期的功能提供了新的思路。PaO与细胞死亡密切相关，无论其是否表现出增加的活性或非活性。
Thermosensitive male sterility plays an important role in wheat fertility and production. As a key enzyme for chlorophyll degradation, pheophorbideaoxygenase (PaO) can suppress cell death in plants. We cloned the wheat geneTaPaO1from the thermosensitive genetic male sterile (TGMS) line BS366; it encodes a typical PaO protein, containing a conserved Rieske [2Fe-2S] iron–sulphur motif, a mononuclear non-heme iron-binding motif, and a C-terminal CxxC motif.TaPaO1was expressed in all tissues and was upregulated during the meiosis stage of BS366 anthers under low temperature. Subcellular localization of TaPaO1 specifically labelled the surrounding of chloroplasts.TaPaO1regulated byRD29Apromoter which responded to low temperature led to pollen sterility in transgenic tobacco. Expression analysis showed thatTaPaO1exhibited a higher level of expression in the anther than in other tissues in transgenic tobacco plants during low temperature treatment. We propose that the higher senescence-related activity ofTaPaO1may lead to the cell death of anthers, which happens at an early developmental stage under low temperature. These results provide new insights into the function of PaO during the early developmental stage of anthers. PaO is closely related to cell death regardless of whether it exhibits increased activity or inactive.