The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface
The phytotoxic activity of the cerato-platanin BcSpl1 resides in a two-peptide motif on the protein surface
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DOI：
10.1111/mpp.12097
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发表时间：
2014-05-01
影响因子：
4.9
通讯作者：
Gonzalez, Celedonio
中科院分区：
文献类型：
--
作者：
Frias, Marcos;Brito, Nelida;Gonzalez, Celedonio
Cerato-platanin家族蛋白是分泌的，并且已经在真菌细胞壁和细胞外培养基中发现。它们在各种植物中引起防御反应，并已被提议被植物免疫系统视为病原体相关分子模式（PAMP），尽管在坏死营养型灰葡萄孢的情况下，Cerato-Platanin BcSpl 1有助于真菌毒力而不是植物抗性。在这项研究中，我们报告说，BcSpl 1，这是以前发现的分泌作为一个丰富的蛋白质，是更丰富的真菌细胞壁。通过融合到绿色荧光蛋白（GFP），我们还表明，BcSpl 1协会与植物质膜引起快速的形态学变化，在细胞水平上，如解体的叶绿体，之前在处理的组织中的宏观坏死。通过一系列的缺失研究，合成肽和嵌合蛋白的组合，我们映射到蛋白质表面的两个肽基序的引发活性。在B中展示该基序的嵌合蛋白的表达。BcSpl 1缺失的灰霉病菌突变体无疑表明，该基序是BcSpl 1对致病力贡献的原因。
Cerato-platanin family proteins are secreted and have been found in both the fungal cell wall and the extracellular medium. They elicit defence responses in a variety of plants and have been proposed to be perceived as pathogen-associated molecular patterns (PAMPs) by the plant immune system, although, in the case of the necrotroph Botrytis cinerea, the cerato-platanin BcSpl1 contributes to fungal virulence instead of plant resistance. In this study, we report that BcSpl1, which was previously found in the secretome as an abundant protein, is even more abundant in the fungal cell wall. By fusion to green fluorescent protein (GFP), we also show that BcSpl1 associates with the plant plasma membrane causing rapid morphological changes at the cellular level, such as the disorganization of chloroplasts, prior to macroscopic necrosis in the treated tissue. By a combination of serial deletion studies, synthetic peptides and chimeric proteins, we mapped the eliciting activity to a two-peptide motif in the protein surface. The expression of a chimeric protein displaying this motif in B. cinerea mutants lacking BcSpl1 undoubtedly showed that the motif is responsible for the contribution of BcSpl1 to virulence.