Genes involved in immunity and apoptosis are associated with human presbycusis based on microarray analysis
Genes involved in immunity and apoptosis are associated with human presbycusis based on microarray analysis
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基于微阵列分析，涉及免疫和细胞凋亡的基因与人类老年性耳聋相关
DOI：
10.3109/00016489.2014.880795
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发表时间：
2014-06-01
影响因子：
1.4
通讯作者：
Shi, Jianrong
中科院分区：
文献类型：
--
作者：
Dong, Yang;Li, Ming;Shi, Jianrong
摘要结论：免疫和细胞凋亡相关基因与老年性聋的发病有关。CCR 3和GILZ可能通过调节趋化因子受体、T细胞凋亡或T细胞活化途径在老年性耳聋的发病中发挥重要作用。目的：筛选与老年性耳聋相关的基因，探讨老年性耳聋的分子发病机制。方法：采用纯音测听法进行听功能测试。使用受试者的外周血样品，通过Illumina Human-6 BeadChip进行微阵列分析以鉴定老年人相关基因。为了识别与老年痴呆症相关基因相关的生物学过程类别和途径，通过基因本体树机器（GOTM）和注释、可视化和集成发现数据库（大卫）进行生物信息学分析。使用定量RT-PCR（qRT-PCR）来验证微阵列数据。结果：基因芯片分析共鉴定出469个上调基因和323个下调基因。基因本体论（GO）分析的优势生物学过程和京都基因与基因组百科全书（KEGG）及BIOCARTA分析的丰富途径均表明参与免疫和凋亡的基因与老年性耳聋相关。此外，CCR 3、GILZ、CXCL 10和CX 3CR 1基因在基因芯片和qRT-PCR数据中显示出一致的组间差异。CCR 3和GILZ在老年性聋患者和正常对照组之间差异有统计学意义（p < 0.05）。
Abstract Conclusions: Genes involved in immunity and apoptosis were associated with human presbycusis. CCR3 and GILZ played an important role in the pathogenesis of presbycusis, probably through regulating chemokine receptor, T-cell apoptosis, or T-cell activation pathways. Objectives: To identify genes associated with human presbycusis and explore the molecular mechanism of presbycusis. Methods: Hearing function was tested by pure-tone audiometry. Microarray analysis was performed to identify presbycusis-correlated genes by Illumina Human-6 BeadChip using the peripheral blood samples of subjects. To identify biological process categories and pathways associated with presbycusis-correlated genes, bioinformatics analysis was carried out by Gene Ontology Tree Machine (GOTM) and database for annotation, visualization, and integrated discovery (DAVID). Quantitative RT-PCR (qRT-PCR) was used to validate the microarray data. Results: Microarray analysis identified 469 up-regulated genes and 323 down-regulated genes. Both the dominant biological processes by Gene Ontology (GO) analysis and the enriched pathways by Kyoto encyclopedia of genes and genomes (KEGG) and BIOCARTA showed that genes involved in immunity and apoptosis were associated with presbycusis. In addition, CCR3, GILZ, CXCL10, and CX3CR1 genes showed consistent difference between groups for both the gene chip and qRT-PCR data. The differences of CCR3 and GILZ between presbycusis patients and controls were statistically significant (p < 0.05).