Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8′-hydroxylase CYP707A
Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8′-hydroxylase CYP707A
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DOI：
10.1016/j.bmc.2010.11.011
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发表时间：
2011-01-01
影响因子：
3.5
通讯作者：
Todoroki, Yasushi
中科院分区：
文献类型：
--
作者：
Okazaki, Mariko;Nimitkeatkai, Hataitip;Todoroki, Yasushi
我们开发了脱落酸（阿坝）8 ′-羟化酶（CYP 707 A）特异性抑制剂阿贝唑-E1（Abz-E1）。该抑制剂被设计和合成为烯效唑（UNI）的放大类似物，烯效唑（UNI）是一种众所周知的植物生长阻滞剂，其抑制赤霉素生物合成酶（对映贝壳杉烯氧化酶，CYP 701 A）以及CYP 707 A。我们的研究结果表明，Abz-E1作为一个有效的抑制剂的CYP 707 A和CYP 701 A的不良抑制剂在体外和体内。施用Abz-E1可提高植物的脱水耐性，并增加内源阿坝。(C)2010爱思唯尔有限公司版权所有。
We developed abscinazole-E1 (Abz-E1), a specific inhibitor of abscisic acid (ABA) 8'-hydroxylase (CYP707A). This inhibitor was designed and synthesized as an enlarged analogue of uniconazole (UNI), a well-known plant growth retardant, which inhibits a gibberellin biosynthetic enzyme (ent-kaurene oxidase, CYP701A) as well as CYP707A. Our results showed that Abz-E1 functions as a potent inhibitor of CYP707A and a poor inhibitor of CYP701A both in vitro and in vivo. Abz-E1 application to plants resulted in improved desiccation tolerance and an increase in endogenous ABA. (C) 2010 Elsevier Ltd. All rights reserved.