Genome-wide characterization of the TALE homeodomain family and the KNOX-BLH interaction network in tomato
Genome-wide characterization of the TALE homeodomain family and the KNOX-BLH interaction network in tomato
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DOI：
10.1007/s11103-022-01277-6
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发表时间：
2022-05-11
影响因子：
5.1
通讯作者：
Mitsuda, Nobutaka
中科院分区：
文献类型：
--
作者：
Ezura, Kentaro;Nakamura, Akiyoshi;Mitsuda, Nobutaka
关键信息全面的酵母和原生质体双杂交分析说明了TALE同源结构域蛋白家族、KNOX和BLH蛋白在番茄叶和果实发育中的蛋白质-蛋白质相互作用网络。属于相同TALE同源结构域家族的KNOTTED样（KNOX，KN）蛋白和BELL 1样（BLH）蛋白通过形成KNOX-BLH异源二聚体模块而一起起作用。这些模块在调节植物的多个发育过程中起着至关重要的作用，如器官分化。然而，尽管对KNOX和BLH功能的了解越来越多，但在大多数植物中，包括番茄（Solanum lycopersicum），一种研究果实和叶发育的重要模式植物，对其功能性蛋白质-蛋白质相互作用（PPI）网络的全面看法仍然难以捉摸。在这里，我们的特点是8个番茄KNOX基因（SlKN 1至SlKN 8）和14个番茄BLH基因（SlBLH 1至SlBLH 14）的表达谱，共表达分析，和PPI网络分析使用双杂交技术在酵母（Y2 H）和原生质体（P2 H）。我们鉴定了75种成对KNOX-BLH相互作用，包括10种主要I类KNOX蛋白SlKN 2/TKN 2的新型相互作用物和9种主要II类KNOX蛋白SlKN 5的新型相互作用物。根据这些数据，我们将KNOX-BLH模块分为几类，这使得我们推断出参与叶和果实分化过程的KNOX-BLH模块的顺序和组合。值得注意的是，共表达和相互作用的SlKN 5和果实优先表达BLH 1-进化枝旁系同源物（SLBLH 5/SLBEL 11和SLBLH 7）表明他们在调节果实分化的重要作用。此外，在计算机模拟的KNOX-BLH模块，序列分析，和P2 H测定确定了几个残基和一个接头区域可能影响BLH的亲和力KNOX在其保守的二聚化结构域。总之，这些发现提供了深入了解番茄器官分化的KNOX-BLH模块的调控机制。
Key message Comprehensive yeast and protoplast two-hybrid analyses illustrated the protein-protein interaction network of the TALE homeodomain protein family, KNOX and BLH proteins, in tomato leaf and fruit development. KNOTTED-like (KNOX, KN) proteins and BELL1-like (BLH) proteins, which belong to the same TALE homeodomain family, act together by forming KNOX-BLH heterodimer modules. These modules play crucial roles in regulating multiple developmental processes in plants, like organ differentiation. However, despite the increasing knowledge about individual KNOX and BLH functions, a comprehensive view of their functional protein-protein interaction (PPI) network remains elusive in most plants, including tomato (Solanum lycopersicum), an important model plant to study fruit and leaf development. Here, we characterized eight tomato KNOX genes (SlKN1 to SlKN8) and fourteen tomato BLH genes (SlBLH1 to SlBLH14) by expression profiling, co-expression analysis, and PPI network analysis using two-hybrid techniques in yeasts (Y2H) and protoplasts (P2H). We identified 75 pairwise KNOX-BLH interactions, including ten novel interactors of SlKN2/TKN2, a primary class I KNOX protein, and nine novel interactors of SlKN5, a primary class II KNOX protein. Based on these data, we classified KNOX-BLH modules into several categories, which made us infer the order and combination of the KNOX-BLH modules involved in differentiation processes in leaf and fruit. Notably, the co-expression and interaction of SlKN5 and fruit preferentially expressing BLH1-clade paralogs (SlBLH5/SlBEL11 and SlBLH7) suggest their important roles in regulating fruit differentiation. Furthermore, in silico modeling of the KNOX-BLH modules, sequence analysis, and P2H assay identified several residues and a linker region potentially influencing the affinity of BLHs to KNOXs within their conserved dimerization domains. Together, these findings provide insights into the regulatory mechanism of KNOX-BLH modules underlying tomato organ differentiation.