Mannitol transport in Streptococcus mutans
Mannitol transport in Streptococcus mutans
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变形链球菌中的甘露醇转运
DOI：
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发表时间：
1975
影响因子：
3.2
通讯作者：
C. Wittenberger
中科院分区：
文献类型：
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作者：
J. Maryanski;C. Wittenberger
在变形链球菌中发现了一个己糖醇诱导的、依赖于磷酸烯醇式丙酮酸的磷酸转移酶系统。从五种变形链球菌的代表性菌株(AHT、BHT、C-67-1、6715和LM7)的甘露醇培养细胞中获得的无细胞提取物能够通过需要磷酸烯醇式丙酮酸和镁离子的反应将甘露醇转化为甘露醇-1-磷酸。从生长在各自底物上的细胞制备的无细胞提取物中发现甘露醇和山梨醇磷酸转移酶活性，但在生长在其他底物上的细胞提取液中均不存在己糖醇磷酸转移酶活性。从葡萄糖生长的细胞中部分纯化了一种耐热的低分子成分，并发现它能刺激甘露醇磷酸转移酶系统。二价阳离子Mn~(2+)和Ca~(2+)部分取代了Mg~(2+)，而锌~(2+)则表现出较强的抑制作用。
A hexitol-inducible, phosphoenolpyruvate-dependent phosphotransferase system was demonstrated in Streptococcus mutans. Cell-free extracts obtained from mannitol-grown cells from a representative strain of each of the five S. mutans serotypes (AHT, BHT, C-67-1, 6715, and LM7) were capable of converting mannitol to mannitol-1-phosphate by a reaction which required phosphoenolpyruvate and Mg2+. Mannitol and sorbitol phosphotransferase activities were found in cell-free extracts prepared from cells grown on the respective substrate, but neither hexitol phosphotransferase activity was present in extracts obtained from cells grown on other substrates examined. A heat-stable, low-molecular-weight component was partially purified from glucose-grown cells and found to stimulate the mannitol phosphotransferase system. Divalent cations Mn2+ and Ca2+ partially replaced Mg2+, while Zn2+ was found to be highly inhibitory.