Evaluation of the effects of four media on human intestinal microbiota culture in vitro
Evaluation of the effects of four media on human intestinal microbiota culture in vitro
复制标题
四种培养基对人肠道菌群体外培养的影响评价
DOI：
10.1186/s13568-019-0790-9
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发表时间：
2019-05-23
期刊：
影响因子：
3.7
通讯作者：
Fang Baishan
中科院分区：
文献类型：
--
作者：
Fu Yousi;Chen Kainan;Fang Baishan
人体肠道菌群在维持人体健康和疾病发病中具有重要作用。本研究的目的是研究4种培养基对人肠道菌群代谢产物和组成变化的影响，我们使用来自3个粪便菌群移植（FMT）供体的肠道菌群样品进行体外分批培养。培养48 h后，肠道微生物群培养基（GMM）具有最高的乙酸（73.00 ± 7.56 mM）和丙酸（16.79 ± 1.59 mM）产量，细菌生长培养基（BGM）具有最高的丁酸产量（13.39 ± 0.56 mM）。此外，脑心浸液（BHI）促进拟杆菌的生长（p < 0.05），尤其是48 h后的拟杆菌，GMM导致放线菌的显著增加（p < 0.05），并增加了双歧杆菌的有益菌群，苛养厌氧菌肉汤（FAB）促进了厚壁菌的繁殖，而BGM促进了土志贺氏菌和阿克曼氏菌的生长。结果表明，4种培养基对人体肠道菌群的代谢和组成有不同的影响。这些结果可以促进来自人类肠道微生物群的细菌的培养。
The human intestinal microbiota has an important role in the maintenance of human health and disease pathogenesis. The aim of this research was to investigate the impact of four media on human intestinal microbiota metabolite and composition changes, we performed in vitro batch culture using intestinal microbiota samples from three fecal microbiota transplantation (FMT) donors. After 48 h culture, gut microbiota medium (GMM) had the highest production of acetic acid (73.00 ± 7.56 mM) and propionic acid (16.79 ± 1.59 mM), bacterial growth media (BGM) had the highest production of butyric acid (13.39 ± 0.56 mM). In addition, brain heart infusion (BHI) promoted (p < 0.05) the growth ofBacteroidetes, especiallyBacteroidesafter 48 h, GMM resulted in a significant increase (p < 0.05) inActinobacteriaand increased the beneficial genusBifidobacterium, fastidious anaerobe broth (FAB) increasedFirmicutespopulation, and BGM promoted the growth ofEscherichia–ShigellaandAkkermansia. The results suggest that four media had different effects on the human intestinal microbiota metabolism and composition in vitro. These results may facilitate the culture of bacteria from the human intestinal microbiota.